水霉菌分离纯化及其有性孢子的诱生技术研究  

Study on Techniques of Isolating,Purifying Culture and Inducement Spores of Saprolegnia Spp

作　　者：张定法[1] 刘鸣韬[1] 李照业[1] 李辉[1] 

机构地区：[1]河南科技学院资环学院,河南新乡453003

出　　处：《河南科技学院学报》2008年第4期30-32,共3页Journal of Henan Institute of Science and Technology(Natural Science Edition)

摘　　要：对水霉菌(Saprolegnia. spp)的分离纯化及其有性孢子的诱生技术进行了研究,筛选出了油菜籽、小麦粒、玉米片、葵花仁、花生仁、辣椒籽等材料作为饵料。其中玉米片诱生水霉菌菌丝体及游动孢子囊所需的时间最短,但易被腐霉菌(Pythium. spp)等杂菌污染;油菜籽诱生菌丝体及游动孢子囊所需时间最长,但菌丝体生长良好并能诱生出有性孢子。先用油菜籽诱出菌丝之后再添加玉米片,有利于藏卵器的形成。菌丝生长及游动孢子囊产生的适宜温度为25℃,藏卵器产生的适宜温度为15℃.Research on techniques of isolating,purifying and inducement spores of Saprolegnia spp,a lots of culture materials,such as seeds of rape,wheat,corn slice,sunflower,peanut and capsicum were selected.Mycelium induced by corn piece grow well in short time than other materials,but easily contaminated by other fungi(Pythium spp.);Mycelium induced by rape seed grow in long time than other materials,but mycelium grow rankly and produced sexual spores easily than in other culture mediums.Zoosporangium were easily i...

关 键 词：水霉菌 分离 纯培养 游动孢子囊 藏卵器 

分 类 号：Q93[生物学—微生物学]