基于复合型微流控芯片的紫外检测毛细管电泳系统  

A Hybrid Microfluidic Chip-based Capillary Electrophoresis System with UV Absorbance Detection

作　　者：曹小丹[1] 方群[1] 方肇伦[1] 

机构地区：[1]浙江大学化学系微分析系统研究所,杭州310028

出　　处：《高等学校化学学报》2004年第7期1231-1234,MJ02,共5页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金重大项目 (批准号 :2 0 2 990 3 0 );国家"八六三"计划项目 (批准号 :2 0 0 2 AA2 Z2 0 42 );教育部科学技术研究重点项目 (批准号 :0 10 93 )资助

摘　　要：提出了一种基于芯片 -毛细管复合装置的紫外检测 -微流控芯片毛细管电泳分析系统 .采用小死体积的耦合技术实现了石英毛细管与“十”字通道型微流控玻璃芯片的耦合 .本系统的紫外检测灵敏度与商品化毛细管电泳仪相当 .采用夹流进样方式 ,达到较高的进样重现性 ,2 mmol/L苯甲酸的峰高相对标准偏差 ( RSD)为 1 .5 % ( n=1 1 ) .A hybrid microfluidic system produced by coupling a microfabricated glass chip with conventional silica capillary for UV absorbance detection was developed. The system has proved to be robust, and easily fabricated at a low cost. The effects of sample loading and separation field strength on plate number, migration time and signal response were observed in the optimization studies. The reproducibility was examined by using benzoic acid as a model sample and following similar behavior as for complete photolithographically produced glass chip systems. The results showed that the reproducibility is good and the RSD(n=11) was only 1.5% for benzoic acid. The performance of the system was demonstrated by separating sulphamethoxazole(SMZ) and trimethoprim(TMP) in sulphatrim tablets within 70 s. The detection limits were comparable to those obtained with a commercial capillary electrophoresis instrument.

关 键 词：复合型微流控芯片 紫外检测 毛细管电泳 复方磺胺甲噁唑 片剂 氧苄氨嘧啶 磺胺甲噁唑 

分 类 号：O657[理学—分析化学] R927.2[理学—化学]