神经节苷脂对人骨髓间充质干细胞诱导分化为神经元样细胞生长状态的影响  

Effect of GM1 on Survival of Neuron-like Cells Differentiate from Human bone marrow mesenchymal stem cells

作　　者：张丽芳[1,2] 杨晓苏[1] 肖波[1] 胡益民[1] 陈冰[1] 彭晶晶[1] 

机构地区：[1]中南大学湘雅医院神经内科,长沙410008 [2]长治医学院附属和平医院

出　　处：《脑与神经疾病杂志》2009年第1期13-16,共4页Journal of Brain and Nervous Diseases

基　　金：湖南省自然科学基金资助项目0JJ3065

摘　　要：目的探讨神经节苷脂对人骨髓间充质干细胞(hMSCs)诱导分化为神经元样细胞后生长状态的影响。方法MSCs由成年人骨髓经密度梯度离心加贴壁培养法获得,取第4～6代MSCs以10ng/ml bFGF预诱导24h后,用丁羟基茴醚(BHA)、二甲基亚砜(DMSO)和不同浓度神经节苷脂(25mmol/L、50mmol/L、75mmol/L)诱导分化,采用免疫细胞化学法鉴定神经细胞特异性抗原标记神经元特异性烯醇化酶(NSE)、微管相关蛋白-2(MAP2)和神经胶质相关蛋白(GFAP)的表达。唑盐比色实验(MTT法)检测不同浓度神经节苷脂对诱导后5h、24h、2d、3d细胞生长情况的影响。结果成功分离培养人MSCs,诱导分化后大部分MSCs变成神经元样细胞并表达NSE和MP2。中等浓度(50mmol/L)神经节苷脂组诱导后的细胞生长活力较低浓度和高浓度组好(P<0.05)。结论中等浓度神经节苷脂能使诱导后的细胞保持较好的生长状态。Objective To study the effect of GM1 on the survival of neuron-like cells differentiated from mesenchymal stem cells in vitro. Methods Human mesenchymal stem cells were isolated primarily from bone marrow of adult volunteers iliac and purified sterilely with gradient centrifugation by Ficoll. The low-density cells including MSCs were cultivated. The 4th to 6th passage of MSCs were induced with bFGF、 DMSO、BHA and different concentrations GM1. The control group did not receive anyinduced treatment. Neuron-spe...

关 键 词：骨髓间充质干细胞 神经元 诱导分化 神经节苷脂 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]