INF-γ/A+874T位点基因多态性与呼吸道合胞病毒毛细支气管炎的相关性  

Association of INF-γ/A+874T gene polymorphisms with respiratory syncytial virus bronchiolitis

作　　者：董琳[1] 黄志英[2] 陈小芳[1] 周晓聪[1] 李锦燕[1] 林剑[1] 

机构地区：[1]温州医学院附属育英儿童医院呼吸科,浙江温州325027 [2]南京医科大学附属常州二院儿科,江苏常州213000

出　　处：《中国当代儿科杂志》2009年第1期21-24,共4页Chinese Journal of Contemporary Pediatrics

基　　金：浙江省医药卫生科技计划(NO:2004B148)

摘　　要：目的干扰素-γ(IFN-γ)分泌不足可能在严重呼吸道合胞病毒(RSV)感染的发病中发挥重要作用。IFN-γ/A+874T基因位点存在多态性,可能与RSV感染的病情存在关联。该研究分析INF-γ/A+874T基因位点在温州地区汉族儿童的分布特征;进一步探讨INF-γ/A+874T位点基因多态性与RSV毛细支气管炎易感性、病情的相关性及对鼻咽分泌物INF-γ和血清总IgE水平的影响。方法采用DNA测序法检测114例住院的汉族RSV毛细支气管炎患儿和90例汉族健康体检儿童INF-γ/A+874T位点基因多态性;采用酶联免疫吸附法测定鼻咽分泌物INF-γ水平,化学发光法测定血清总IgE水平。结果两组均存在INF-γ/A+874T位点基因多态性,以AA纯合子基因型为主,其中RSV毛细支气管炎组AA、AT基因型频率分别为82.5%,17.5%,对照组分别为AA77.8%,AT21.1%,两组差异无显著性意义;病例组等位基因频率分别为A90.4%,T9.6%,对照组分别为A88.3%,T11.7%,两组差异均无显著性意义。轻度和中重度患儿INF-γ/+874位点2种基因型的差异亦无显著性意义。病例组2种基因型鼻咽分泌物INF-γ及血清总IgE水平差异均无显著性意义。结论温州地区汉族儿童存在INF-γ/A+874T位点基因多态性,但未发现该位点变异与RSV毛细支气管炎易感性、病情、鼻咽分泌物INF-γ及血清总IgE水平存在关联。Objective A deficient interferon-gamma (IFN-gamma) response has been involved in the pathogenesis of severe respiratory syncytial virus (RSV) infection. Gene polymorphisms in IFN-gamma/A+874T have been associated with the susceptibility to asthma and might be related to disease severity of RSV infection. This study investigated the single nucleotide polymorphisms (SNPs) of IFN-gamma/A+874T in Han children in Wenzhou area and to explore the correlation between gene polymorphisms of IFN-gamma/A +874T and the ...

关 键 词：呼吸道合胞病毒 毛细支气管炎 干扰素-Γ 基因多态性 儿童 

分 类 号：R725.6[医药卫生—儿科]