葡聚六糖对烟草悬浮培养细胞活性氧的作用  

Effect of Active Oxygen in Tobacco Suspension Cultures Induced by Glucohexaose

作　　者：孙艳秋[1] 李宝聚[2] 石延霞[2] 陈捷[3] 

机构地区：[1]沈阳农业大学植物保护学院,沈阳110161 [2]中国农业科学院蔬菜花卉研究所,北京100081 [3]上海交通大学农业与生物学院,上海201101

出　　处：《吉林农业大学学报》2009年第1期37-40,共4页Journal of Jilin Agricultural University

基　　金：国家自然科学基金项目(30270910);北京市自然科学基金重点项目(6021004)

摘　　要：研究了葡聚六糖对烟草悬浮培养细胞活性氧的作用。结果表明:葡聚六糖可以诱导烟草悬浮细胞活性氧的迸发,活性氧迸发峰值出现在30 min,同时活性氧相关酶系活性也升高,超氧化物歧化酶(SOD)活性最高峰在处理后60 min,过氧化物酶(POD)和过氧化氢酶(CAT)变化趋势与活性氧相似。添加不同抑制剂EG-TA、新霉素、氯化锂(LiCl)等测定发现,细胞胞内和胞外Ca2+参与调控葡聚六糖诱导的活性氧迸发。Active oxygen could be induced to burst by glucohexaose in tobacco suspension cultures,and oxidative burst maximum was in 30 minutes,and relative enzyme activity of active oxygen was also improved in cells.The maximum of SOD activities was in 60 minutes,and the current of POD and CAT activities changes were similar to active oxygen.By adding different inhibitors such as EGTA,neomycin,LiCl and so on,intracellar and extracellar Ca2+ were indicated to be able to take part in regulating active oxygen burst indu...

关 键 词：葡聚六糖 烟草悬浮细胞 活性氧 

分 类 号：S572[农业科学—烟草工业]