毓麟合剂的HPLC指纹图谱研究  

HPLC FingerPrints of Yu-Lin mixture

作　　者：张玉萱 年华 徐玲玲 

机构地区：[1]岳阳中西医结合医院药剂科

出　　处：《内蒙古中医药》2009年第2期46-47,共2页Inner Mongolia Journal of Traditional Chinese Medicine

基　　金：上海中医药大学附属岳阳中西医结合医院院内课题

摘　　要：目的:采用HPLC建立毓麟合剂的指纹图谱测定方法。方法:采用ZORBAXSB-C18柱(4.6×150mm,5μm)色谱柱,甲醇-0.01mol/L盐酸水溶液为流动相进行梯度洗脱,流速0.5mL/min,检测波长250nm。结果:建立了毓麟合剂的HPLC对照指纹图谱,共检出14个分离度良好的共有峰。结论:所选择的10批毓麟合剂指纹图谱具有良好的相似性,该方法可靠、简便,可作为毓麟合剂质量控制的参考方法。AIM:To set up the fingerprint chromatograms of Yu-Lin mixture by HPLC.METHODS:The chromatographic seperation was performed on an ZORBAX SB-C18 column(4.6×150mm,5μm)with a mobile phase consisting of methanol-0.01 mol/L hydrochloric acid water,gradient eluent,at the flow rate of 0.5 mL/min,detection wavelength was set at 250 nm.RESULT: Set up the fingerprint chromatograms of Yu-Lin mixture by HPLC,we have pinpointed 14 common peaks.CONCLUTION:There are good similarities among 10 batches of Yu-Lin mixture,and ...

关 键 词：毓麟合剂 质量控制 指纹图谱 HPLC 

分 类 号：R286[医药卫生—中药学]