RAPD技术对白花蛇舌草真伪鉴别的应用  

Identification of Spreading Hedyotis Herb by RAPD

作　　者：李坚韧[1] 王红艳[2] 康熙雄[2] 

机构地区：[1]北京急救中心,100031 [2]首都医科大学附属北京天坛医院,100050

出　　处：《中国误诊学杂志》2009年第3期509-510,共2页Chinese Journal of Misdiagnostics

摘　　要：目的:探讨RAPD技术在鉴别白花蛇舌草的应用价值。方法:采用RAPD技术,在PCR技术的基础上建立随机扩增多态性DNA技术,对5个不同产地的白花蛇舌草及其伪混品水线草进行了DNA指纹鉴定。结果:白花蛇舌草与水线草的DNA指纹图谱有明显不同,而不同产地的白花蛇舌草的DNA指纹图谱基本一致。结论:该方法简单、快速、重复性好,可用于白花蛇舌草与其伪混品如水线草等的鉴别。Objective:To establish a RAPD method to indentify spreading hedyotis herb.Methods:The DNA fingerprints of spreading hedyotis herb from five localities and Hedyotis corymbosa(L.)Lam.from two regions was made for identification by the RAPD technique in this study.Results:The established DNA fingerprints of spreading hedyotis herb and its confusion variety were obviously different,but those of the different regions were the same.Conclusion:The method is simple,rapid,and reproductive and suitable for identifica...

关 键 词：白花蛇舌草/分离和提纯 随机扩增多态DNA技术 

分 类 号：R284[医药卫生—中药学]