抗心肌肌凝蛋白重链自身抗体IgG亚类对腹主动脉缩窄术后大鼠左室功能的影响  

Effect of anti-cardiac myosin heavy chain auto-antibodies IgG subclass on left ventricular function in rats by coarctation of abdominal aorta

作　　者：莫新玲[1] 谢福生[1] 黄文新[1] 阳耀忠[1] 严冬雪[1] 夏中华[1] 

机构地区：[1]桂林医学院附属医院心血管内科,广西桂林541001

出　　处：《大连医科大学学报》2009年第1期53-57,共5页Journal of Dalian Medical University

基　　金：广西壮族自治区卫生厅课题(Z2007230)

摘　　要：[目的]探讨抗心肌肌凝蛋白重链自身抗体(AMCHA)IgG亚类的产生与腹主动脉缩窄术后大鼠左室功能的关系。[方法]应用腹主动脉缩窄术建立慢性心力衰竭(CHF)大鼠模型,测定血流动力学变化,计算左心室重量/体重(LVW/BW)比值;以合成的大鼠心肌肌凝蛋白重链部分肽段(1135-1150氨基酸残基)为包被抗原,采用间接ELISA方法检测大鼠血清中心肌肌凝蛋白重链自身抗体(MCHA)IgG亚类的水平及动态变化。[结果]CHF大鼠手术组与假手术组相比左室压力上升和下降最大速率降低(+dp/dtm ax:2103.91±606.89 vs 3196.64±383.29;-dp/dtm ax:-1709.60±460.96 vs-2762.64±514.42,P<0.01),左室舒张末压升高(LVEDP:17.49±8.33 vs-5.69±1.19,P<0.01)、左室收缩压升高(LVSP:131.67±25.14 vs 111.98±4.63,P<0.05)、左心室重量/体重比值升高(LVW/BW:3.04±0.44 vs 2.57±0.20,P<0.01),术后1~2周,IgG1、IgG2 a和IgG2b自身抗体...[Objective] To study whether there is an anti-cardiac myosin heavy chain auto-antibodies(AMHCA) IgG subclass in serum of rats by coarctation of abdominal aorta,and to investigated whether there was any relationship between AMCHA and the left ventricular function.[Methods] The hemodynamics parameters were determinated and LVW/BW were calculated,meanwhile anti-cardiac myosin heavy chain auto-antibodies(AMCHA) IgG subclass level and dynamic variation were detected by Indirect-ELISA assay using synthetic cardi...

关 键 词：心肌肌凝蛋白 自身抗体 左室功能 

分 类 号：R541.6[医药卫生—心血管疾病]