复方谷氨酰胺肠溶胶囊中L-谷氨酰胺释放度的测定  

Determination of drug release of glutamine in its enteric coated capsules

作　　者：董得时[1] 谭爱萍[1] 汤新强[1] 

机构地区：[1]大连医科大学附属第一医院药剂科,辽宁大连116011

出　　处：《大连医科大学学报》2009年第1期114-117,共4页Journal of Dalian Medical University

摘　　要：[目的]采用HPLC法测定复方谷氨酰胺肠溶胶囊中L-谷氨酰胺的释放度。[方法]采用NUCLEOSIL 5 NH21OOA(250 mm×4.6 mm)色谱柱,乙腈-0.05 mol.L-1磷酸二氢钾缓冲液(pH4.0,70∶30)为流动相,流速1 mL.min-1,检测波长215 nm。以转篮法测定其释放度。[结果]L-谷氨酰胺在0.5~4μg范围内,色谱峰面积与对照品浓度呈现良好的线性关系,r=0.9999;日内精密度高中低3个浓度RSD分别为1.72%、0.62%、0.97%;加样回收率分别为98.0%(RSD=2.13%,n=5),99.0%(RSD=1.60%,n=5)。[结论]该方法简便准确,重现性好,结果准确,可以用于该制剂释放度的测定。[Objective] To establish HPLC method for the determination of drug release of L-Glutamine in its enteric-coated capsules.[Methods] Used NUCLEOSIL 5 NH2 100A column(250 mm 4.6 mm),acetonitrile-0.05 mol L-1 potassium di-hydrogen phosphate buffer(pH 4.0,70∶30)as mobile phase at a flow rate of 1mL min-1,the detection wave-length was 215 nm.Drug release was determined by rotating basket method.[Results] The calibration curves were linear in the range of 0.5～4 μg(r=0.9999).The within-day precision of three thickn...

关 键 词：L-谷氨酰胺 高效液相色谱 释放度 

分 类 号：R927[医药卫生—药学]