5-HT_(2A)受体对延髓面神经后核内侧区呼吸节律的调控作用  

Role of 5-HT2A receptors in regulation of respiratory rhythm in medial region of nucleus retrofacialis

作　　者：何国军[1] 黄明[2] 卢少军[1] 赖福生[1] 吴中海 

机构地区：[1]解放军第454医院神经科,江苏南京210002 [2]空军雷达学院宜昌训练大队卫生队,湖北宜昌443000 [3]南方医科大学基础医学院生理教研室,广东广州510515

出　　处：《东南国防医药》2009年第1期6-9,13,共5页Military Medical Journal of Southeast China

基　　金：国家自然科学基金(30570670);广东省自然科学基金(5004714)

摘　　要：目的探讨5-HT2A受体在延髓面神经后核内侧区(the medial region of nucleus retrofac-ialis,mNRF)对呼吸节律调控的作用。方法仿Suzue方法制作新生大鼠含有舌下神经根及mNRF的离体延髓脑片标本,以吸附电极记录舌下神经根呼吸节律性放电活动(the respiratory rhythmicdischarge activity,RRDA)并作为呼吸活动的指标,采用全细胞膜片钳记录模式在mNRF同步记录呼吸神经元。分别观察1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane(DOI)、ketanserine对mNRF呼吸起步神经元及RRDA的影响。结果DOI可显著使呼吸周期缩短、放电峰值增加,ketanserine使呼吸周期延长、放电峰值降低;DOI可使Cd2+非敏感性呼吸起步神经元发放幅度、时间、spike的频率显著增加,ketanserine则使其显著减少;voltage steps(电压阶跃)和ramps(斜坡电压法)表明ketanserine可抑制Cd2+非敏感性呼吸起步神经元的短暂性和持久性的钠电流。结论5-HT2A受体兴奋对RRDA、Cd2+非敏感性呼吸起步神经元具有兴奋性作用,是通过调节钠电流而起作用的。Objective To explore the role of 5-HT2A receptors in regulation of respiratory rhythm in the medial region of nucleus retrofacialis(mNRF).Methods Medullary slices of neonatal SD rats,including hypoglossal nerve(Ⅻn) and mNRF,were made according to Suzue s method.Simultaneous recording of the Ⅻn respiratory rhythmic activity(RRDA) with suction electrode and the respiratory neuronal discharge were performed with whole cell patch in the mNRF on the brainstem slice in vitro.The effect of 1-(2,5-dimethoxy-4-iodop...

关 键 词：呼吸中枢 延髓 面神经后核 内侧区 5-HT2A 呼吸节律 

分 类 号：R332.3[医药卫生—人体生理学]