电针对抑郁大鼠5-羟色胺和乙酰胆碱酯酶在海马表达的影响  

Influence of Electroacupuncture of "Baihui"(GV 20) and "Sanyinjiao"(SP 6) on Hippocampal 5-HT and AChE Immuno-activity in Chronic Depression Rats

作　　者：朱艺[1] 刘群英[1] 卓廉士[1] 

机构地区：[1]重庆医科大学中医药学院,重庆401331

出　　处：《针刺研究》2009年第1期16-20,共5页Acupuncture Research

摘　　要：目的:探究电针治疗抑郁症的作用机制。方法:将健康Wistar大鼠随机分为正常组10只,模型组和电针组各11只,慢性应激法21 d造模。电针组选穴"百会""三阴交",以频率2 Hz、电流强度1 mA,行电针治疗20 min,每日1次。14 d后处死动物,通过免疫组化法测定5-羟色胺(5-HT)和乙酰胆碱酯酶(AChE)在海马区的积分吸光度(IOD)值。结果:造模后造模组与正常组比较,水平、垂直运动次数和糖水消耗量均减少(P<0.001)。模型组与正常组相比,5-HT和AChE的IOD值减小(P<0.05);电针组与模型组相比,两指标的IOD值明显升高(P<0.05)。结论:抑郁大鼠海马5-HT和AChE活性降低,电针可明显提高大鼠海马中5-HT、AChE的表达,从而发挥其抗抑郁作用。Objective To investigate the influence of electroacupuncture(EA) on 5-serotonin(5-HT) and acetyl cholinesterase(AChE) activities in hippocampus tissue in chronic stressed-induced depression rats.Methods Thirty-two Wistar rats were randomized into normal control group(n=10),model group(n=11) and EA group(n=11).Chronic depression model was established by lonely raising,and chronic unpredictable mild stress for 21 days.EA(2 Hz,1 mA) was applied to "Baihui"(GV 20) and "Sanyinjiao"(SP 6) for 20 minutes,once dail...

关 键 词：抑郁症 电针 海马 5-羟色胺 乙酰胆碱酯酶 

分 类 号：R245[医药卫生—针灸推拿学]