妊娠浓度的雌激素对诱导CD4^+CD25^-naive T细胞转化为CD4^+CD25^+Treg细胞的影响  

Conversion of CD4^+CD25^-naive T cells to CD4^+CD25^+ regulator T cells by pregnant level estrogen

作　　者：汪理[1] 林星光[1] 金昊[1] 童荔[1] 陈忠华 昌盛[1] 

机构地区：[1]华中科技大学附属同济医院器官移植研究所国家教育部/卫生部重点实验室

出　　处：《免疫学杂志》2009年第1期61-63,67,共4页Immunological Journal

基　　金：国家自然科学基金面上项目(30500468)资助

摘　　要：目的体外观察妊娠浓度的雌激素能否诱导CD4+CD25-naive T细胞转化为CD4+CD25+Treg细胞,并探讨其相关性。方法以CD4+CD25-T细胞作为反应细胞,实验组加入妊娠水平的雌激素(E2)及CD3/CD28单抗作为刺激原培养72 h,设阴性对照组(仅加入CD3/CD28单抗)和空白对照组。72 h后检测各组中CD4+CD25+T细胞和CD4+Foxp3+T细胞比例变化及Foxp3 mRNA表达。结果1)阴性对照组CD4+CD25+T细胞比例较空白对照组显著增高(P<0.001),而实验组CD4+CD25+T细胞比例进一步升高(P<0.001)。2)阴性对照组不能诱导CD4+Foxp3+T细胞比例增高,但实验组CD4+Foxp3+T细胞的比例则较其它2组均显著升高(P<0.001)。3)RT-PCR提示阴性对照组Foxp3 mRNA的表达量较空白对照组无显著差异(P>0.05);而实验组Foxp3 mRNA的表达量较其它2组均显著升高(P<0.001)。结论体外淋巴细胞刺激实验提示妊娠状态下雌激素的高水平与CD4+CD25+Foxp3+Treg细胞比例的升高密切相关。Objective To investigate whether pregnant level estrogen can induce CD4+CD25-naive T cells to convert into CD4+CD25+ regulator T cells in vitro.Methods CD4+CD25-naive T cells were stimulated with anti-CD3 mAb and anti-CD28 mAb in the presence or absence of pregnant level estrogen(E2)(experimental group and control group),while CD4+CD25-naive T cell co-cultured with PBS was taken as placebo group.72 hours after stimulation,the changes of CD4+CD25+ T cells and CD4+Foxp3+ T cells were detected and the expressi...

关 键 词：妊娠 雌激素 CD4+CD25+调节性T细胞 Foxp3 

分 类 号：R392.1[医药卫生—免疫学]