高致病性人禽流感H5N1疫苗滴鼻免疫应答效果的初步研究  

Immunity response induced by intranasal immunization with inactived human high pathogenic avian influenza vaccine

作　　者：何蕊[1] 吕进[1] 张良艳[1] 董梅[1] 王希良[1] 

机构地区：[1]军事医学科学院微生物流行病研究所病原微生物生物安全国家重点实验室

出　　处：《免疫学杂志》2009年第1期16-19,共4页Immunological Journal

基　　金：国家防控禽流感专项课题(2004BA519A70)

摘　　要：目的通过高致病性人禽流感H5N1全病毒-MF59佐剂疫苗滴鼻免疫Balb/c小鼠,评价该疫苗所诱导的系统免疫与黏膜免疫应答效果。方法以不同剂量抗原按比例与MF59佐剂配伍制成粘膜疫苗,滴鼻免疫Balb/c小鼠,二免2周采血检测血清IgG、IgM效价及血清中HAI(HAinhibitor)的中和抗体效价,同时收集鼻、肺灌洗液,检测其IgG和sIgA抗体效价。结果H5N1+MF59组血清抗体效价较H5N1组有显著升高(P<0.01);在各剂量组中,随着剂量的增加抗体效价呈上升趋势,12μg后抗体效价呈下降趋势,以H5N1+MF59(12μg)组效价最高;肺鼻灌洗液中,均可检测到特异性分泌型IgA、IgG,其中特异性分泌型IgA效价略高于IgG;抗体亚型的分布以IgG1、IgG2b为主。结论灭活高致病性禽流感全病毒H5N1在佐剂MF59作用下可诱导机体产生体液免疫应答,同时还可以在黏膜局部产生特异性分泌型IgA、IgG,为高致病人禽流感病毒H5N1黏膜疫苗的研制奠定了基础。Objective To study the systemic and mucosal antibody response induced by intranasal immunization with inactived high pathogenic avian influenza vaccine combined with adjuvant MF59.Methods Inactived high pathogenic avian influenza vaccine formulated with MF59 was administered intranasally.Mouse sera were collected every week after immunization.Changes of serum IgG,IgA,and IgM were observed,as well as the titers of hemagglutination inhibition(HAI) and the specific IgG and sIgA in lung and nasal washes were de...

关 键 词：高致病性人禽流感H5N1疫苗 滴鼻免疫 免疫应答 

分 类 号：R392.7[医药卫生—免疫学]