山东地区冠状动脉粥样硬化性心脏病患者3号染色体基因扫描研究  

A genomic screen on chromosome 3 in coronary heart disease in Shandong Province

作　　者：谢毅[1] 郭媛[1] 陈刚[2] 李继福[1] 朱海宁[2] 魏然[2] 王博[2] 栾萌[2] 

机构地区：[1]山东大学齐鲁医院心内科,济南250012 [2]山东省医学科学院基础医学研究所医学遗传学与基因工程重点实验室,济南250062

出　　处：《山东大学学报（医学版）》2009年第1期56-60,共5页Journal of Shandong University:Health Sciences

基　　金：山东省科技攻关项目(2007GG20002025)

摘　　要：目的对冠心病患者及正常对照者的3号染色体进行扫描,查找与冠心病关联的遗传位点。方法在3号染色体上采用间隔为10 cM(厘摩)遗传距离的23个微卫星遗传标记,分别对156例冠心病患者和450名正常对照者的DNA混合池样本进行基因组扫描,采用CLUMP软件对比患者组与对照组等位基因频率的差异。结果在D3S1278(3q13.31)位点和D3S1300(3p14.2)位点患者组与对照组的等位基因频率差异有统计学意义(P<0.001)。结论山东地区冠心病患者群体中存在与3号染色体q13.31和p14.2区域的关联,致病基因或调控因子可能位于其附近。Objective A screen on chromosome 3 in coronary heart disease(CHD) patients and normal controls from Shandong Province was performed to find the genetic loci associated with CHD.Methods A total of 23 microsatellite markers on chromosome 3 spaced at approximate 10 cM were used in screening of two separated DNA pool samples consisting of 156 CHD cases and 450 normal controls.Statistic analysis was performed by CLUMP software to compare the difference in allele frequency of each locus between the two pooled sam...

关 键 词：冠状动脉疾病 3号染色体 遗传 基因组 基因频率 DNA混合池 

分 类 号：R541.4[医药卫生—心血管疾病]