HPLC-ELSD法测定阿拉伯糖的含量  

Determination of Arabinose by HPLC-ELSD

作　　者：刘峥[1] 崔小兵[1] 李伟[1] 徐智勇[1] 

机构地区：[1]南京中医药大学,江苏南京210029

出　　处：《现代中药研究与实践》2009年第1期37-40,共4页Research and Practice on Chinese Medicines

摘　　要：目的建立高效液相色谱-蒸发光散射检测法测定阿拉伯糖含量的方法。方法采用SHISEIDONH2分析色谱柱(4.6mm×250mm,5μm),柱温:30℃,流动相:乙腈-水(70:30),流速:0.8mL/min,ELSD参数:漂移管温度为80℃,N2流速为2.0L/min。结果阿拉伯糖在12.00～27.99μg范围内与峰面积呈良好的线性关系(r=0.9999),平均回收率为100.4%,RSD=0.8%,定量限为200μg/mL(S/N=10)。结论HPLC-ELSD法简便,快捷,无干扰,灵敏度高,重现性好,可用于阿拉伯糖的含量测定。Objective To establish a HPLC-ELSD method for the determination of arabinose.Methods The analytical column was SHISEIDO NH2(4.6 mm×250 mm,5 μm).The column temperature was 30℃.The mobile phase was acentonitrile-water(70:30).The flow rate was 0.8 mL/min.The samples were determined by an evaporative light-scattering detector(ELSD) with the drift tube temperature at 80 ℃ and the flow rate of nitrogen was 2.0 L/min.Results The linear range of arabinose was 12.00～27.99 μg(r=0.999 9).The average recovery was 100.4...

关 键 词：高效液相色谱-蒸发光散射检测法 阿拉伯糖 含量测定 

分 类 号：R284.1[医药卫生—中药学]