二鳖散对大鼠Walker-256肝癌肿瘤生长及血清VEGF,endostatin表达的影响  

Effects of Erbie San on Walker-256 liver cancer and adjustment to unbalance of VEGF/endostatin in rats

作　　者：王淑美[1] 徐晓玉[2] 张文亮[3] 李傲[4] 董伟[4] 

机构地区：[1]重庆医科大学中医药学院,重庆401331 [2]西南大学药学与中医药学院,重庆400715 [3]重庆医科大学附属第一医院,重庆400016 [4]重庆医科大学药学院,重庆400016

出　　处：《中国中药杂志》2009年第1期89-91,共3页China Journal of Chinese Materia Medica

基　　金：重庆市卫生局项目(200730)

摘　　要：目的:探讨二鳖散对大鼠Walker-256肝癌肿瘤生长及血清血管内皮生长因子(VEGF)、内皮抑素(Endostatin)表达的影响,从抗血管生成角度探讨二鳖散的抗癌作用。方法:采用Walker-256癌肉瘤腹水接种Wistar大鼠制作肝癌模型,随机分为二鳖散低、中、高剂量(1.25,2.5,5g·kg-1·d-1)组、5-氟脲嘧啶75mg·kg-1组及模型组。于造模的第9天开始灌胃,每日1次,共21d。检测各组大鼠瘤重、肿瘤抑制率,ELISA法检测各组大鼠血清VEGF,endostatin的表达,计算比较各组VEGF/endostatin的比值。结果:二鳖散中、高剂量组和5-氟脲嘧啶组大鼠瘤重减轻,其血清VEGF表达量降低,Endostatin表达量升高,VEGF/endostatin比值降低,与模型组比较,差异有显著性(P<0.05或P<0.01)。结论:二鳖散能抑制大鼠Walker-256肝癌的生长,其机制可能与调控VEGF/endostatin的失衡而抑制肿瘤血管生成有关。Objective:To investigate the anticancer effects of erbie San on the rats bearing Walker-256 liver cancer and the potential mechanism of its angiogenesis effects. Method:Wistar rats bearing Walker-256 liver cancer were used in this study. The experimental groups were treated with Erbie San 1.25,2.5,5 g·kg-1·d-1,and 5-Fluorouracil injection 75 mg·kg-1,respectively. The tumor s weight,the expression of VEGF,Endostatin and the ratio of VEGF /endostatin in serum of each groups were observed. Result:Compared to t...

关 键 词：二鳖散 Walker-256肝癌 血管内皮生长因子 内皮抑素 

分 类 号：R285.5[医药卫生—中药学]