内蒙地区蒙汉族儿童过敏性紫癜HLA-A、B关联基因的探讨及测序分析  

Investigation and sequencing analysis on the HLA-A and B-relevant genes in Henoch-purpura of children of Mongolian and Han populations in internal Mongolia region

作　　者：任少敏[1] 杨光路[1] 刘春枝[1] 张春霞[1] 于少飞[1] 李维才[1] 

机构地区：[1]内蒙古医学院附属医院儿科,呼和浩特010050

出　　处：《现代免疫学》2009年第1期68-71,共4页Current Immunology

摘　　要：探索内蒙地区蒙、汉族儿童过敏性紫癜(AP)与HLA-A、B等位基因的关联性及易感基因是否有基因突变位点。在祖籍三代居住内蒙地区,无血缘关系,无与异族通婚史及其他免疫性疾病史和家族史的蒙、汉族人群中,分别选择儿童AP蒙族56例、汉族50例为病例组,正常健康儿童蒙族66名、汉族96名为对照组。引入PCR-SSO技术,分析HLA-A、B等位基因的多态性,并将易感基因进一步做SBT高分辨分型及DNA序列分析。结果显示:(1)蒙古族病例组HLA-A*11和HLA-B*15基因频率均较对照组明显增高(P<0.05 OR>1,其95%可信区间内均不包含1,EF>0),高分辨后,前者均为A*1101,后者主要是B*1501,其余为B*15**。而HLA-B*07和HLA-B*40基因频率均较对照组明显降低(P<0.05 OR<1,其95%可信区间内均不包含1,EF>0);(2)汉族病例组HLA-A*26、HLA-B*35基因频率均较对照组明显增高(P<0.05 OR>1,其95%可信区间内均不包含1,EF>0),高分辨后,前者均为A*2601,后者主要是B*3503,其余为B*35**。蒙汉族病例组均未发现基因突变位点。由此可得出HLA-A*11和HLA-B*15可能是内蒙地区蒙古族儿童AP发病的2个遗传易感基因;而HLA-B*07和HLA-B*40为其保护基因;而HLA-A*26和HLA-B*35为汉族儿童AP发病的遗传易感基因。The relationship of the HLA-A and B-relevant genes and the Henoch-purpura in children of Mongolian and Han population in internal Mongolian region as well as the sequencing analysis were investigated.In the present study,56 Mongolian children and 50 Han population children with anaphylactoid purpura(AP) were selected for study,whose ancestral homes in three generations were all situated at internal Mongolian region,having no any blood-relationship and without history of mixed marriages or family history of ...

关 键 词：过敏性紫癜 儿童 蒙古族 汉族 HLA-A、B基因 PCR-SSO PCR-SBT 

分 类 号：R72[医药卫生—儿科]