6-氨基-5-氰基-4-(2-羟基)苯基-3-甲基-1-苯基吡啶[2,3-c]并吡唑与牛血清白蛋白相互作用的研究  

The interaction between 6-amino-5-cyano-4-(2-hydroxy)phenyl-3-methal-1-phenylpyridino[2,3-c]pyrazole and bovine serum albumin

作　　者：张立新[1] 廉淑芹[2] 

机构地区：[1]徐州师范大学化学化工学院,江苏徐州221116 [2]徐州医学院药学院,江苏徐州221004

出　　处：《徐州师范大学学报（自然科学版）》2009年第1期80-83,共4页Journal of Xuzhou Normal University(Natural Science Edition)

摘　　要：采用紫外光谱及荧光光谱法研究了6-氨基-5-氰基-4-(2-羟基)苯基-3-甲基-1-苯基吡啶[2,3-c]并吡唑(1f)与牛血清白蛋白(BSA)的相互作用,发现静态猝灭和非辐射能量转移是引起荧光猝灭的主要原因.在292,298,307 K,它们之间的结合常数(KA)分别为2.71×104,2.41×104,2.62×104L.mol-1,结合位点数(n)为1.13,1.15,1.09.根据Forster非辐射能量转移机理,测得了1f与BSA相结合距离(r)及能量转移效率(E).结果表明,它们之间的结合力为疏水作用力.The interaction between 6-amino-5-cyano-4-(2-hydroxy)phenyl-3-methal-1-phenylpyridino[2,3-c]pyrazole(1f) and bovine serum albumin(BSA) was investigated by using fluorescence(FS) and ultraviolet spectroscopy(UV).It is found that static action and non-radiation energy transfer are the main reasons for the fluorescence quenching.At 292,298 and 307 K,the apparent binding constants(KA) between 1f and BSA are 2.71×104,2.41×104,2.62×104 mol-1,and the binding sites(n) are 1.13,1.15,1.09.According to the Forster the...

关 键 词：6-氨基-5-氰基-4-(2-羟基)苯基-3-甲基-1-苯基吡啶[2 3-c]并吡唑 牛血清白蛋白 荧光光谱法 

分 类 号：R96[医药卫生—药理学]