上胸段硬膜外阻滞对兔基底动脉痉挛后内皮素-1和内皮型一氧化氮合酶基因表达的影响  

Effects of high thoracic epidural block on expressions of ET-1 and eNOS following basilar artery vasospasm in rabbits

作　　者：林剑清[1,2] 陈国忠[1] 刘韧[1] 吴晓智[1] 江鹤群[1] 吴坛光[1] 

机构地区：[1]南京军区福州总医院麻醉科,350025 [2]厦门大学附属中山医院麻醉科,361004

出　　处：《实用医学杂志》2009年第5期705-707,共3页The Journal of Practical Medicine

摘　　要：目的:建立兔上胸段硬膜外阻滞(HTEB)模型和蛛网膜下腔出血(SAH)模型,探讨预先HTEB对兔基底动脉痉挛后内皮素-1(ET-1)和内皮型一氧化氮合酶(eNOS)基因表达的影响。方法:切开置管法制备HTEB模型的新西兰兔30只,随机分为正常组(N组)、单纯SAH组(S组)和HTEB+SAH组(HS组)3组,每组10只。HS组硬膜外以0.1%罗哌卡因1mL/h持续给药至实验完毕。用枕大池穿刺一次注血法(0.5mL/kg)制成兔SAH模型。饲养7d,用经颅多普勒(TCD)监测第7天兔基底动脉平均血流速度(Vm);用RT-PCR方法测定基底动脉preproET-1mRNA和eNOSmRNA的表达。结果:实验第7天TCD显示,S组的Vm较N组明显升高(P<0.05),HS组与N组组间无明显变化(P>0.05)。preproET-1mRNA的表达,与N组相比,S组(P<0.01)和HS组(P<0.05)均增高,S组高于HS组(P<0.05);eNOSmRNA的表达,与N组相比,S组降低(P<0.01),HS组差异无显著性(P>0.05),S组低于HS组(P<0.01)。结论:预先HTEB可减轻SAH后脑血管痉挛的程度,并可引起兔基底动脉内皮细胞下调preproET-1mRNA和上调eNOSmRNA的表达水平。Objective To investigate the effects of preemptive high thoracic epidural block(HTEB) on the expressions of ET-1 and eNOS following basilar artery vasospasm in rabbits.Methods Thirty rabbits were randomly divided into 3 groups:normal group(group N), SAH group(group S), and SAH group receiving HTEB by a continuous epidural injection of 0.1% ropivacaine 1 mL / h(group HS).Transcranial Doppler ultrasonography was used to detect the basilar artery blood flow velocity(Vm)changes on day 7 of establishment of the ...

关 键 词：血管痉挛 颅内 麻醉 硬膜外 基底动脉 兔 

分 类 号：R614[医药卫生—麻醉学]