急性心肌损伤激活脊髓水平白细胞介素1β信号通路  

Acute cardiac injury activates interleukin-1β signaling in the spinal cord

作　　者：刘建华[1] 傅迪[2] 徐军美 卢向航[1] 戴茹萍[1] 

机构地区：[1]中南大学湘雅二医院麻醉科,长沙410011 [2]中南大学湘雅医院麻醉科,长沙410008

出　　处：《中南大学学报（医学版）》2009年第3期210-215,共6页Journal of Central South University ：Medical Science

基　　金：国家自然科学基金(30700790);湖南省科技厅基金(2007tp4006)~~

摘　　要：目的:探讨急性心肌损伤对脊髓水平IL-1β信号通路表达的影响。方法:采用心肌内注射5%甲醛建立急性心肌损伤模型,于术后1,3,6,24及72h取颈部脊髓进行研究。Western印迹检测急性心肌损伤后各个时间段脊髓水平IL-1β的蛋白表达量。免疫组织化学和原位杂交检测IL-1β的细胞定位。电泳迁移率(EMSA)试验检测急性心肌损伤对IL-1β的下游转录因子NF-κB和AP-1活性的影响。结果:急性心肌损伤后,脊髓水平的IL-1β的表达显著增高并且持续至心肌损伤后72h;上调的IL-1β的蛋白和mRNA主要在脊髓灰质II～IV层神经元中表达;急性心肌损伤后,NF-κB和AP-1的DNA结合率显著增强。结论:急性心肌损伤导致脊髓水平IL-1β信号通路的激活,激活的中枢神经系统的细胞因子通路有可能进一步加重心肌损伤。Objective To investigate the effect of acute cardiac injury on the activation of interleukin-1β(IL-1β) signaling in the spinal cord.Methods Acute cardiac injury rat model was established by intra-myocardial injection of formalin through diagphragm.IL-1β expression was determined by Western blot,immunohistochemistry and in situ hybridization.The DNA binding activities of 2 IL-1β transcription factors,activator protein(AP)-1 and nuclear factor kB(NF-κB) were measured by electrophoretic mobility shift assay(EM...

关 键 词：心肌损伤 脊髓 白细胞介素-1Β 核转录因子-ΚB 激活蛋白-1 

分 类 号：R541[医药卫生—心血管疾病]