硫化铅纳米颗粒标记DNA电化学探针的研究  

Study on lead sulfide nanopaticle labeled electrochemical DNA probe

作　　者：祝宁宁[1] 

机构地区：[1]上海师范大学生命与环境科学学院,上海200234

出　　处：《上海师范大学学报（自然科学版）》2004年第2期65-69,共5页Journal of Shanghai Normal University(Natural Sciences)

摘　　要：在水溶液中合成了表面具有自由羧基的硫化铅(PbS)纳米颗粒,以乙基-(3-二甲基丙基)碳二亚胺盐酸盐(EDAC)为偶联活化剂,将其标记于人工合成的5′端氨基修饰的寡聚核苷酸(ODN)片段上,制备成PbS纳米颗粒标记DNA探针。在一定的条件下,使其与固定在玻碳电极表面的待测DNA序列进行杂交反应,利用阳极溶出示差脉冲伏安法间接测定Pb(Ⅱ)的量,从而实现对互补、非互补DNA片段的识别和电化学检测。同时对该探针的稳定性、选择性进行了讨论。Synthesis of lead sulfide (PbS)nanoparticles and its application in electrochemical DNA assay were described. The nan-oparticles were successfully labeled to a 5- amino group capped oligonucleotides probe. The DNA assay relies on the target oligo-nucleotides on the glassy carbon electrode (GCE) hybridization of the PbS nanoparticle-oligonucleotides DNA probe. Hybridization events between probe and target were monitored by the release of the PbS anchored on the hybrids by oxidative dissolution and the indirectly determination of the solubilized Pb2+. The detection limit is 10 pmol/L of the complementary target oligonucleotides.

关 键 词：硫化铅纳米颗粒 电化学DNA探针 DNA杂交 

分 类 号：O657[理学—分析化学]