人软骨细胞中褪黑素受体mRNA的表达及意义  

Expression and significance of melatonin receptor mRNA in human chondrocyte

作　　者：孙光权[1] 邱勇[1] 朱泽章[1] 黄爱兵[1] 李海波[1] 刘臻[1] 

机构地区：[1]南京大学医学院附属鼓楼医院脊柱外科,210008

出　　处：《江苏医药》2009年第2期156-158,120,共4页Jiangsu Medical Journal

基　　金：江苏省卫生厅重大项目(K200610)

摘　　要：目的检测人软骨细胞中褪黑素受体mRNA的表达,并探讨其意义。方法取8例手术切除的人髂软骨组织进行软骨细胞培养,并用Ⅱ型胶原免疫组化对培养的细胞进行软骨细胞鉴定。取二代软骨细胞抽提总RNA,用逆转录聚合酶链反应(RT-PCR)检测褪黑素受体mRNA的表达。结果人软骨细胞中总RNA以MT1和MT2两种受体引物进行的RT-PCR,产物电泳呈阳性条带;其基因片段长度与MT1和MT2受体引物之间的核酸长度相当。结论人软骨细胞中存在MT1、MT2两种褪黑素受体mRNA的表达,证明软骨细胞是褪黑素作用的靶细胞,褪黑素可能通过存在于软骨细胞中的MT1、MT2受体来调节骨骼的生长发育。Objective To study the expression and significance of melatonin receptor mRNA in human chondrocyte.Methods The chondrocytes from 8 human iliac growth-plate were cultured in monolayer and passaged.Immunohistochemical stain of collagen Ⅱ was used to confirm the chondrocyte phenotype.Chondrocyte was collected for extracting total RNA and the mRNA of melatonin receptor was detected by reverse transcript-polymerase chain reaction(RT-PCR) method.Results The electrophoretic results of RT-PCR products by MT1 and MT...

关 键 词：褪黑素受体 软骨细胞 

分 类 号：R68[医药卫生—骨科学]