荧光探针法研究PNA-DNA双链和dsDNA结构的差异  

Study on the otherness of the configurations between PNA-DNA and DsDNA by fluorescence probe

作　　者：周海清[1] 沈鹤柏[1] 周文骏 杨永桃[1] 

机构地区：[1]上海师范大学生命与环境科学学院,上海200234 [2]上海兽药饲料监测所,上海201103

出　　处：《上海师范大学学报（自然科学版）》2004年第2期78-82,共5页Journal of Shanghai Normal University(Natural Sciences)

基　　金：上海市自然科学基金资助项目(02ZA14068)

摘　　要：运用荧光和紫外吸收光谱研究了Ru(bipy)2(dppz)2+和苯胺红T两种荧光试剂与PNA-DNA、dsDNA之间的相互作用。实验结果表明:相同条件下同一荧光试剂与PNA-DNA、dsD-NA两者之间发生不同的作用,从而说明了PNA-DNA、dsDNA的结构具有一定的差异,并对引起差异的原因进行了初步的探讨。Ru(bipy)2 (dppz)2+ and Safranine T are used as the fluorescence probe. The interactions of two fluorescent molecules. Ru(bipy)2(dppz)2+ and Safranine T, on PNA-DNA and dsDNA are studied respectively. When the same fluorescent molecular reacts with these two different targets. the distinct fluorescence is observed under the same conditions. This experimental results indicate that the interaction between the probe and PNA-DNA is different from that between the probe and dsDNA. There must be some differences between PNA-DNA and dsDNA in structure. UV spectra was also used to confirm our conclusions.

关 键 词：荧光探针 PNA 插入键合作用 PNA—DNA 荧光光谱 UV 

分 类 号：O644.17[理学—物理化学]