中药复方益肾蠲湿合剂抑制肾小球系膜细胞增殖及机理的研究  

Inhibition Effect of "Yishenjuanshi" Traditional Chinese Herbs on Proliferation of Glomerular Mesangial Cell and the Mechanisms Involved

作　　者：孙影[1] 张雪鹏[2] 张健[3] 杨方[1] 

机构地区：[1]华北煤炭医学院病理教研室,河北唐山063000 [2]华北煤炭医学院附属医院外三科,河北唐山063000 [3]华北煤炭医学院中西医结合医院,河北唐山063000

出　　处：《辽宁中医药大学学报》2009年第4期202-204,共3页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：唐山市科学技术局资助(04362001B-1)

摘　　要：目的:观察自拟中药复方"益肾蠲湿合剂"对肾小球系膜细胞(glomerular mesangial cell,GMC)增殖的影响,并进一步探讨其作用机理。方法:将1×10-3、1×10-2和1×10-1mg/mL的"益肾蠲湿合剂"提取液作用于体积分数10%血清的DMEM培养的GMC,分别用MTT和Western Blot方法检测细胞的增殖和磷酸化ERK1/2的表达。结果:1×10-2和1×10-1mg/mL"益肾蠲湿合剂"可显著抑制GMC的增殖,抑制率在12、24和36 h分别为13.4%、为9.6%。细胞总ERK的表达在各组间无明显差别。但不同浓度的"益肾蠲湿合剂"能显著地抑制血清所诱导的ERK1/2的磷酸化,最高抑制率达68%。结论:"益肾蠲湿合剂"可通过抑制ERK1/2的磷酸化来抑制GMC的增殖。Objective: To observe the effect of"Yishenjuanshi"traditional Chinese herbs on the proliferation of glomerular mesangial cell(GMC) and the possible mechanisms involved.Methods:All herbs involved in Yishenjuanshi"are immersed in ethanol to isolate the viable components which are dissolved in DMSO.After cultured in serum free medium for 48hs,GMC are stimulated by 10% FSC with or without Yishenjuanshi"treatment to detect GMC proliferation and expression of phosphorylated extracellular regulated protein kinases...

关 键 词：益肾蠲湿合剂 肾小球系膜细胞 增殖 ERK 

分 类 号：R285.5[医药卫生—中药学]