中药材中西维因残留量的薄层色谱法测定  

Determination of Carbaryl Residue in Chinese Herbs by Thin Layer Chromatography with Fluorescence Detection

作　　者：胡秋菊[1] 朱若华[1] 苏文斌[1] 强红[1] 王香凤[1] 谷学新[1] 

机构地区：[1]首都师范大学化学系,北京100037

出　　处：《首都师范大学学报（自然科学版）》2004年第2期38-40,45,共4页Journal of Capital Normal University：Natural Science Edition

摘　　要：本文研究了中药材西维因残留量的薄层色谱 荧光测定 .采用环已烷∶丙酮∶氯仿 =5∶1∶1 (V V)作为展开剂 ,直接用荧光测定 .本法灵敏度高 ,且易操作 ,当添加水平为 0 0 1mg mL时 ,回收率在 84 %～ 1 1 1 %之间 ,线性相关系数为 0 9998,检测限达到 1 0ng .A method for determination of carbaryl residue in Chinese herbs by thin layer chromatography with fluorescence detection was studied.The samples were developed by cyclohexane:actone:chloroform=5∶1∶1( V/V) and were directly measured by fluorescence emission.The method had high sensitivity and was easy to be operated.As spiked level was 0 01?mg/mL,the recovery was between 84% and 111%,the correlation coefficient was 0.999?8,the detection limit was 10?ng.

关 键 词：中药材 西维因 残留量 薄层色谱法 测定 氨基甲酸酯类农药 

分 类 号：R284.1[医药卫生—中药学]