人参总皂苷合黄连小檗碱对慢性心衰大鼠血浆BNP、心肌细胞内钙离子浓度的影响  

Effects of ginseng total saponins with berberine on plasma brain natriuretic peptide and Ca^(2+) concentration in experimental rats with chronic congestive heart failure

作　　者：李永民[1] 陈晓春[1] 刘华[1] 罗飞[1] 李刚[1] 

机构地区：[1]河北北方学院中医系,河北张家口075029

出　　处：《中国中药杂志》2009年第3期324-327,共4页China Journal of Chinese Materia Medica

基　　金：河北省科技支撑计划课题(08276101D-65)

摘　　要：目的:研究人参总皂苷(ginseng total saponins,GS)合黄连小檗碱(berberine,Ber)对慢性心衰大鼠血流动力学、血浆脑钠肽(BNP)、心肌细胞内钙离子浓度[Ca2+]i的影响。方法:连续12 d皮下注射较大量异丙肾上腺素(ISO)制备慢性心衰大鼠模型,ISO总量80 mg.kg-1,随机分为4组:模型组,每天蒸馏水灌胃;GS+Ber组(各20 mg.kg-1)、卡托普利组(45 mg.kg-1),每天灌胃1次;参麦注射液组(5 mL.kg-1),每天腹腔注射1次。另设正常组,每天蒸馏水灌胃。连续给药4周,测定各组大鼠血流动力学变化、血浆BNP含量,流式细胞仪测定心肌细胞内[Ca2+]i。结果:与正常组相比,模型组左心室内压峰值(LVSP)、左心室等容收缩期压力上升最大速率(+dp/dtmax)、左心室舒张期压力下降最大速率(–dp/dtmax)明显下降,左心室舒张末压(LVEDP)、血浆BNP、心肌细胞内[Ca2+]i显著升高,均有显著性差异(P<0.01);与模型组相比,GS+Ber组LVSP,±dp/dtmax明显升高,LVEDP、血浆BNP、心肌细胞内[Ca2+]i显著降低,均有显著性差异(P<0.01,P<0.05),治疗效果接近卡托普利组,优于参麦注射液组。结论:GS+Ber对慢性心衰大鼠血流动力学异常、血浆BNP、心肌细胞内钙超载有良好改善作用。Objective:To study the effects of total saponins of Panax ginseng combined with berberine on hemodynamics,Plasma brain natriuretic peptide and Ca2+ concentration in cardiac cells of chronic congestive heart failure rats.Method:Animal models of heart failure were induced by subcutaneously injecting a large dose of ISO(80 mg.kg-1)for 12 days Rat and then were randomly divided into four groups:model group was given distilled water by gavage;total saponins of P.ginseng combined with berberine group was treated ...

关 键 词：人参总皂苷 黄连小檗碱 心力衰竭 血流动力学 脑钠肽 钙离子 

分 类 号：R285[医药卫生—中药学]