基于寒/热性对照抗原的斑点免疫印迹法中药药性研究  

Research on medical speciality of traditional Chinese medicines using dot-immunoblotting method based on polyclonal antibody prepared from traditional Chinese medicines with hot /cold nature

作　　者：王厚伟[1] 窦彦玲[1] 田景振[1] 李峰[1] 王世军[1] 王振国[1] 

机构地区：[1]山东中医药大学药学院,山东济南250355

出　　处：《中国中药杂志》2009年第4期438-442,共5页China Journal of Chinese Materia Medica

基　　金：国家重点基础研究发展计划(973)项目(2007CB512601);山东省优秀中青年科学家奖励基金(2007BSB14087)

摘　　要：目的:从免疫原性上探讨中药药性的物质基础。方法:以高良姜、肉桂与仙茅3味典型热性中药水提物的混合品作为热性对照抗原,以黄连、大黄与知母3味典型寒性中药水提物的混合品作为寒性对照抗原,免疫家兔后,制备相应多克隆抗体,分别应用寒、热性对照抗原抗体与9味中药水提物作斑点免疫印迹,杂交信号应用QuantityOne定量扫描分析。结果:9味中药与热性对照抗原多克隆抗体的印迹信号大小顺序为:干姜、附子、杜仲、秦皮、金银花、知母、黄连、大黄、黄柏;与热性对照抗原多克隆抗体的印迹信号相似度分别为:57.33%,43.56%,34.16%,30.2%,28.81%,26.53%,21.68%,17.62%,14.85%;9味中药与寒性对照抗原多克隆抗体的印迹信号大小顺序为:大黄、知母、黄连、黄柏、干姜、金银花、秦皮、杜仲、附子;与寒性对照抗原多克隆抗体的印迹信号相似度分别为:55.22%,54.23%,46.72%,34.08%,30.3%,24.48%,24.33%,20.35%,15.17%。以印迹信号相似度为聚类变量,经Wistar’sMethod聚类分析,9味中药被聚为2大类,一大类为:黄柏、知母、黄连、大黄,该大类与寒性对照抗原距离小,与寒性对照抗原的距离依次增大;另一大类为:干姜、附子、杜仲、秦皮、金银花,该大类与热性对照抗原距离小,与热性对照抗原的距离依次增大。结论:总体上,测试中药与对照抗原抗体的印迹信号相似度越小,其药性与对照抗原的距离越大;斑点免疫印迹法是中药药性物质基础研究的一种实用、有效的新方法。Objective:To research on the substantial foundation of the medical speciality of Chinese traditional medicines from immunogenicity.Method:Control antigen with hot nature was prepared from the mixture of the aqueous extracts of three Chinese traditional medicines with three typical hot nature of Alpinia officinarum,Cinnamomum cassia and Curculigo orchioides,while that with cold nature prepared with Rheum palmatum,Anemarrhena asphodeloides,Coptis chinensis,and polyclonal antibody was prepared by immunizing ra...

关 键 词：寒/热性中药 多克隆抗体 斑点免疫印迹 

分 类 号：R285[医药卫生—中药学]