高效液相色谱法同时测定乌药中的4种生物碱  

Simultaneous determination of four alkaloids in Lindera aggregate by high performance liquid chromatography

作　　者：韩铮[1] 苏慧丽[1] 陈娜[1] 栾连军[1] 吴永江[1] 

机构地区：[1]浙江大学药学院现代中药研究所,浙江杭州310058

出　　处：《中国中药杂志》2009年第5期583-586,共4页China Journal of Chinese Materia Medica

摘　　要：目的:建立同时测定乌药中4种生物碱的HPLC法。方法:Agilent ZORBAX SB-C18色谱柱(4.6 mm×250 mm,5μm);流动相A相为水(先加入1.5‰的二乙胺,再用冰醋酸调pH 3.0),B相为乙腈,线性梯度洗脱;UV检测波长289 nm。结果:波尔定碱在0.428~8.56μg,降波尔定碱在2.122~31.83μg,网状香荔枝碱在0.760~15.20μg,linderegatine在0.020 4~0.400 8μg与峰面积呈良好的线性关系,平均加样回收率为99.18%,100.94%,100.31%,99.17%,RSD分别为1.7%,2.5%,2.1%,3.0%。结论:该方法快速简便、准确,可用于乌药的质量评价。Objective: To develop an HPLC method for simultaneous determination of four major alkaloids in Lindera aggregate.Method: The analysis was carried out on an Agilent ZORBAX SB-C18 column(4.6 mm×250 mm,5 μm) with gradient elution using acetonitrile-water(containing 0.15% diethylamine,adjusted to pH=3.0 with acetic acid) as mobile phase.Flow rate was 1.0 mL·min-1 and the detection wavelength was at 289 nm.Result: The calibration curves were linear over the range of 0.428-8.560 μg for boldine,2.122-31.83 μg for ...

关 键 词：乌药 生物碱 含量测定 高效液相色谱法 

分 类 号：R284[医药卫生—中药学]