羟基红花黄色素A对裸鼠人胃腺癌BGC-823移植瘤血管及VEGF,bFGF mRNA表达的影响  

Effects of hydroxy safflor yellow A on blood vessel and mRNA expression with VEGF and bFGF of transplantation tumor with gastric adenocarcinoma cell line BGC-823 in nude mice

作　　者：奚胜艳[1] 张前[1] 解华[1] 刘林涛[1] 刘朝阳[2] 高学敏[1] 张建军[1] 吴立坤[1] 钱丽丽[1] 邓晓迎[1] 

机构地区：[1]北京中医药大学基础医学院,北京100029 [2]中国医学科学院肿瘤医院肿瘤研究所,北京100021

出　　处：《中国中药杂志》2009年第5期605-610,共6页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(30572436)

摘　　要：目的:研究羟基红花黄色素A(HSYA)对人癌裸鼠皮下移植瘤血管生长、血管内皮生长因子(vascular endothelialgrowth factor,VEGF)及碱性成纤维生长因子(basic fibroblast growth factor,bFGF)mRNA表达的影响。方法:运用BALB/C nu/nu裸小鼠接种人胃腺癌细胞株BGC-823于右前肢腋部皮下建立裸鼠人癌移植瘤模型,随机分为模型组、阳性药对照组、HSYA高、低剂量组,模型组给予生理盐水腹腔注射,HSYA两组分别给予0.056,0.028 g.L-1HSYA溶液腹腔注射,此3组均每只每日注射2次,间隔4~6 h,每次0.2 mL;阳性药对照组给予2 g.L-1环磷酰胺(cytoxan,CTX)每只腹腔注射0.2 mL,每2天1次。20 d后,检测各组肿瘤体积、重量,光镜观察瘤组织病理改变,实时荧光定量PCR(real time quantitative PCR,RT-qPCR)检测瘤组织VEGF和bFGF mRNA的表达。结果:HSYA0.028 g.L-1组移植瘤体积(607.42±252.96)mm3、质量(0.88±0.14)g,VE...Objective: To investigate the effects of Hydroxy Safflor yellow A(HSYA) on the growth of blood vessel of transplantation tumor of gastric adenocarcinoma cell line BGC-823 in nude mice and its underlying mechanism of antagonizing tumor angiogenesis.Method: The BGC-823 cells was subcutaneouly injected into the right anterior armpit of BALB/C nu/nu nude mice and established the animal model of transplantation tumor.Then nude mice were divided into 4 groups at random: model group,control group,high or low dosag...

关 键 词：羟基红花黄色素A BGC-823移植瘤 裸鼠 血管内皮生长因子 碱性成纤维生长因子 肿瘤血管生成 

分 类 号：R285.5[医药卫生—中药学]