黄芪总苷及黄芪甲苷对糖皮质激素诱导老前期大鼠记忆损伤的保护作用及机制  

Protective effects of AST and ASI on memory impairment and its mechanism in senescent rats treated by GC

作　　者：李维祖[1] 李卫平[1] 尹艳艳[1] 公惠玲[1] 吴国翠[1] 朱芬芳[1] 

机构地区：[1]安徽医科大学药理教研室,安徽合肥230032

出　　处：《中国中药杂志》2009年第2期199-203,共5页China Journal of Chinese Materia Medica

基　　金：安徽省自然科学基金项目(00144414);安徽省高校省学术带头人科学研究项目(2005hbz18)

摘　　要：目的:探讨黄芪总苷(astragaloside,AST)及黄芪甲苷(astragalus saponin I,ASI)对糖皮质激素诱导老前期大鼠记忆障碍的延缓作用及对胸腺和海马神经元胞浆钙[Ca^(2+)]_i和凋亡的影响。方法:用氧化可的松((hydroconsonc,HC,10 mg·kg^(-1),sc,21 d)建立老前期大鼠的衰老模型,用Y迷宫检测大鼠的学习记忆功能:在体外用地塞米松(dexamethasonc,DEX,1μmol·L^(-1))诱导胸腺细胞和海马神经元,Fura-2/AM负载法检测大鼠胸腺细胞和海马神经元[Ca^(2+)]_i,琼酯糖凝胶电泳检测DNA梯度和流式细胞仪检测凋亡情况。结果:与模型组比较,AST和ASI能改善HC诱导老前期大鼠衰老模型组大鼠的记忆功能,增加大鼠体重、脑指数和胸腺指数;AST和ASI能够抑制DEX诱导的胸腺细胞和海马神经元[Ca^(2+)]_i的升高和凋亡。结论:AST和ASI对HC诱导老前期大鼠记忆障碍具有延缓作用,其机制可能与抑制海马神经元[Ca^(2+)]_i升高和凋亡有关。Objective:To study the protective effects and mechanisms of astragaloside (AST) and astragalus saponin 1 (ASI) on the memory impairment in senescent rats treated by glucocorticoid (GC).Method:Y maze test was performed to determine the effects of AST and ASI on memory impairment of hydrocortisone(HC)-induced senescent rats.Using Ca^(2+) sensitive fluorescent indicator (Furo-2),free intracellular calcium concentration ([Ca^(2+)]_i) was measured by double wavelength fluorescence sepectrophotometer in thymocyte...

关 键 词：黄芪总苷 糖皮质激素 胞浆钙 凋亡 

分 类 号：R285.5[医药卫生—中药学]