蛇葡萄素对K562/ADR细胞耐药性的逆转作用及机制研究  

Reversal effect and its mechanism of ampelopsin on multidrug resistance in K562/ADR cells

作　　者：叶建涛[1] 郑义磊[1] 刘德育[1] 

机构地区：[1]中山大学药学院,广东广州510089

出　　处：《中国中药杂志》2009年第6期761-765,共5页China Journal of Chinese Materia Medica

基　　金：广东省科技计划项目(2003C104033)

摘　　要：目的:研究蛇葡萄素与阿霉素合用对人白血病多药耐药细胞株K562/ADR增殖的抑制作用及其机制。方法:MTT法测定蛇葡萄素与阿霉素合用对K562/ADR细胞的细胞毒作用,应用金正均公式进行联合用药效果分析;藻红蛋白标记抗体检测蛇葡萄素对K562/ADR细胞膜表面P-糖蛋白表达的影响;流式细胞术测定蛇葡萄素对K562/ADR细胞内阿霉素累积的影响。结果:1.25~5 mg.L-1的蛇葡萄素能够明显逆转K562/ADR细胞对阿霉素的耐药性。1.25 mg.L-1蛇葡萄素与低浓度阿霉素合用可表现出拮抗效应,而浓度在2.5 mg.L-1以上的蛇葡萄素与阿霉素合用可表现出相加至协同效应。蛇葡萄素能够浓度依赖性减少K562/ADR细胞膜P-糖蛋白表达,增加细胞内阿霉素的浓度。结论:蛇葡萄素能通过抑制P-糖蛋白外排药物,促进抗癌药物在细胞内的累积,增强抗癌药物对耐药细胞的细胞毒作用,逆转肿瘤多药耐药。Objective:To investigate the inhibitory effect of ampelopsin(AMP) combined with adriamycin(ADR) on growth of human leukemia multidrug resistant cell line K562/ADR.Method:MTT assay was used to detect the effect of AMP on the cytotoxicity of ADR.Jin s formula was used to analyze the effect of combined drug therapy.The expression of P-glycoprotein(P-gp) on cell membrane of K562/ADR was detected using PE-labeled antibody.Flow cytometry was used to determine the influence of AMP on the intracellular accumulation...

关 键 词：蛇葡萄素 白血病 多药耐药 P-糖蛋白 K562/ADR细胞 

分 类 号：R285.5[医药卫生—中药学]