高效液相色谱法测定盐酸奈福泮片的含量  

Determination the Content of Nefopam Hydrochloride Tablets by HPLC

作　　者：孙东凯[1] 陈建[2] 

机构地区：[1]天津市宝坻区药品检验所,天津301800 [2]天津市药品检验所

出　　处：《中国药师》2009年第2期197-198,共2页China Pharmacist

摘　　要：目的:建立盐酸奈福泮片中盐酸奈福泮含量的HPLC测定方法。方法:采用Dikma C_(18)柱(150mm×4.6mm,5μm),以0.01mol·L^(-1)庚烷磺酸钠溶液(稀磷酸调节pH为3.0)-乙腈(67:33)为流动相,流速:1.0ml·min^(-1);检测波长215nm;柱温: 40℃。结果:线性范围为5～35μg·ml^(-1)(r=0.999 8);平均回收率为98.9%,RSD为0.7%(n=6)。结论:方法操作简便,快速准确。Objective:To determine the content of nefopam hydrochloride tablets by HPLC.Method:The Dikma C_(18) column (150mm×4.6mm,5μm) was used,the 0.01mol·L^(-1) sodium heptanesulfonate solution(Adjusted to pH 3.0 with dilute phosphoric acid)-acetonitrile(67:33) as mobile phase,the flow rate was 1.0 ml·min^(-1),the detection wavelegth was 215nm and the column temperature was 40℃.Result:The linear range of nefopam hydrochloride was 5-35μg·ml^(-1)(r=0.999 8),the average recovery was 98.8%with RSD 0.7%(n=6).Conclusion:...

关 键 词：高效液相色谱法 盐酸奈福泮 含量测定 

分 类 号：R927.2[医药卫生—药学]