TNF-α和LPS对培养大鼠BBBEC胞间通讯的影响  

The effect of TNF-αand LPS on intercellular communication of blood brain barrier endothelial cells

作　　者：刘洁晓[1] 蒲传强[1] 郝延磊[1] 

机构地区：[1]中国人民解放军总医院神经内科,北京100853

出　　处：《脑与神经疾病杂志》2008年第5期581-584,共4页Journal of Brain and Nervous Diseases

基　　金：国家自然基金课题资助(No39670267)

摘　　要：目的:观察体外培养的血脑屏障内皮细胞(blood brain barrier endothelial cells,BBBEC)之间是否存在缝隙连接及在TNF-α和LPS刺激下BBBEC胞间通讯的改变。方法:利用荧光染料CFDA进行负载,在CLSM下用荧光光淬灭恢复技术观察BBBEC间缝隙连接介导的胞间通讯情况,并检测TNF-α和LPS对BBBEC胞间通讯的影响。结果:正常体外培养的BBBEC经荧光淬灭后,30s内出现荧光恢复,至120s达到高峰。与对照组相比,不同浓度TNF-α刺激BBBEC后,其胞间通讯的降低均有显著性意义(P<0.01);但在不同浓度TNF-α之间,胞间通讯的改变无显著性差异(P>0.05)。与对照组相比,低浓度(1μg/ml)LPS不能显著地降低BBBEC的胞间通讯(P>0.05);但高浓度LPS可显著降低细胞间通讯(P<0.01)。结论:体外培养的BBBEC间存在缝隙连接。TNF-α和LPS均可抑制BBBEC缝隙连接介导的胞间通讯。Objective:To investigate interendothelial junctions and intercellular communication of blood brain barrier endothelial cells(BBBCE)stimulated by TNF-αand LPS.Methods:Using Fluo-3/AM and CFDA,influence to [Ca2+]i and intercellular communication of cultured RBMEC by TNF-αand LPS were observed by Confocal Laser Scanning Microscope(CLSM).Results:After photobleaching by fluorescence,cultured BBBCE resume at 30 seconds and reach a peak at 120 seconds.Compared to the contrast,intercellular communication of BBBCE l...

关 键 词：血脑屏障 细胞间通讯 内皮细胞 内毒素 肿瘤坏死因子 

分 类 号：Q42[生物学—神经生物学]