蜗牛肝脏β-甘露聚糖酶的分离提取及纯化鉴定  

THE ISOLATION,ABSTRACTION,PRIFICATION AND IDENTIFICATION OF β-MANNANASE FROM SNAIL LIVER

作　　者：赵娜[1] 王和平[1] 蔺日胜[1] 任旭荣[1] 杨逸隆[1] 

机构地区：[1]内蒙古农业大学生物工程学院,呼和浩特010018

出　　处：《内蒙古农业大学学报（自然科学版）》2008年第4期141-146,共6页Journal of Inner Mongolia Agricultural University(Natural Science Edition)

基　　金：国家自然科学基金(39870558)

摘　　要：从蜗牛肝脏组织制备粗酶液后,经硫酸铵分级分离和两次聚丙烯酰胺凝胶制备电泳,纯化到了蜗牛肝脏β-甘露聚糖酶。该酶经SDS-PAGE电泳分析显示为单一条带,其表观相对分子量为37KD。本法纯化的蜗牛肝脏β-甘露聚糖酶,比活力达到992.3U/mg,提纯倍数达8.45倍,回收率为25.12%。Extracting healthy liver tissue from snails,preparing of rough enzyme liquid,and is purified by twice(NH4)2SO4 precipitation and twice PAGE purification,getting purified ?-mannanase.The molecular weight of the enzyme is 37 KD,an activity reached 992.3 U/ mg,purification multiplier is 8.45 times and the recovery is 25.12%.

关 键 词：蜗牛 Β-甘露聚糖酶 制备电泳 

分 类 号：Q814.1[生物学—生物工程]