白藜芦醇对人皮肤癌细胞株A431生长抑制机制实验研究  

Resveratrol on the Growth of Human Skin Cancer Cell Line A431 and Its Underlying Mechanism

作　　者：焦慧琴[1] 王蕤[1] 孙海梅[1] 刘永[1] 郭敏[1] 苏红星[1] 周德山[1] 

机构地区：[1]首都医科大学基础医学院组织学与胚胎学教研室

出　　处：《首都医科大学学报》2009年第2期212-217,共6页Journal of Capital Medical University

基　　金：国家自然科学基金资助项目(30871310)~~

摘　　要：目的探讨白藜芦醇(resveratrol,Res)对人皮肤癌细胞株A431和正常角质形成细胞株HaCaT体外生长的影响及其机制;并与顺铂(DDP)比较,评价Res作为天然抗肿瘤药物的潜在价值。方法培养A431细胞和HaCaT细胞,使用不同浓度Res或单独DDP处理后,采用四甲基偶氮唑蓝(MTT)法检测其对细胞的增生抑制率;相差显微镜下观察细胞的形态改变;流式细胞术分析细胞凋亡情况;免疫组织化学染色法检测Bcl-2和Caspase-3蛋白。结果①Res和DDP均能明显抑制A431细胞的增生,并呈时间——剂量依赖性;在各自相应半数抑制浓度(IC50),DDP对HaCaT细胞的增生抑制率高于Res(P<0.05);②镜下观察可见Res处理后A431细胞呈现凋亡的形态特征;流式细胞检测发现A431细胞的凋亡呈时间——剂量依赖性;免疫组织化学染色可见Bcl-2蛋白着色减弱、Caspase-3蛋白表达增强。结论Res对A431的增生具有一定的抑制作用,且不良反应较DDP弱;Res也可诱导A431发生凋亡,可能与抑制Bcl-2表达、促进caspase-3表达有关。Objective To investigate the effects of Res on the growth of skin cancer cell line A431 and benign epidermal keratinocyte cell line HaCaT in human and its mechanism.In addition,to evaluate the potential value of Res as a natural antitumor drug.Methods A431 and HaCaT cells were incubated in HDMEM medium containing 10% serum and 1% SP,then they were each treated with different concentrations of Res and DDP.MTT assay was used to measure the inhibitory rate.Phase contrast microscope was used to observe the morp...

关 键 词：白藜芦醇 顺铂 A431细胞株 皮肤癌 BCL-2 CASPASE-3 

分 类 号：R285.5[医药卫生—中药学]