槐白皮对辐射损伤小鼠免疫调节及骨髓造血细胞的修复作用  

Recovery Effects of Sophora Japonical on Immunoregulation and Bone Marrow Hematopoietic Cells in Mice after Irradiation

作　　者：张婵[1] 陈向敏[1] 董海艳[1] 李安乐[1] 

机构地区：[1]温州医学院,浙江温州325027

出　　处：《现代临床医学》2009年第2期94-96,共3页Journal of Modern Clinical Medicine

基　　金：浙江省教育厅科研项目SJY03011(20030800);温州医学院科研项目XNK07055

摘　　要：目的:探讨槐白皮对急性放射损伤小鼠免疫调节及骨髓造血细胞的修复作用。方法:取50只昆明小鼠,随机分为槐白皮组、照射对照组和正常组。采用MTT法检测小鼠脾淋巴细胞IL-2水平及免疫细胞化学SABC染色法检测骨髓组织中VEGF的表达水平。结果:IL-2水平检测槐白皮0.2 mg.mL-1、0.4 mg.mL-1、0.8 mg.mL-1浓度组与照射对照组比较有显著性差异(P<0.05),与正常组比较无显著性差异(P>0.05);且槐白皮浓度0.4 mg.mL-1组最强,槐白皮浓度的0.2 mg.mL-1为最弱。槐白皮各浓度组的VEGF表达水平与照射对照组比较有显著性差异(P<0.05),槐白皮浓度为0.4 mg.mL-1浓度组与正常组比较无显著性差异(P>0.05)。结论:槐白皮可调节免疫功能,增强抗辐射能力,对急性放射损伤小鼠骨髓造血细胞的恢复具有促进作用。Objective:To explore the recovery effects of sophora japonical on immunoloregulation and bone marrow hematopoietic cells in mice after irradiation.Methods:50 mice were divided into 3 groups: sophora japonical group、irradiation injured group and normal group.The proliferation of T lymphocyte and the level of IL-2 were determined by MTT procedure.The level of VEGF was detected by immuohistochemical staining in the bone marrow of mice.Results:It was found that the level of IL-2 in sophora japonical group was s...

关 键 词：槐白皮 免疫调节 IL-2 VEGF 

分 类 号：R285.5[医药卫生—中药学]