体外表达的Glypican-3的CTL表位肽诱导特异性免疫应答  

Specific immune responses induced by CTL epitope peptides of Glypican-3 expressed in vitro

作　　者：王媛媛[1] 肖冰[1] 王文静 徐伟文[2] 李明松[1] 

机构地区：[1]南方医科大学南方医院消化内科 [2]南方医科大学生物技术学院

出　　处：《免疫学杂志》2009年第2期122-124,共3页Immunological Journal

基　　金：国家973计划(2007CB512901);广东省自然科学基金(06105111);广东省科技攻关计划(0700016)资助

摘　　要：目的探讨体外表达的Glypican-3(GPC3)能否诱导出GPC3特异性的细胞免疫应答。方法体外表达GPC3的CTL表位肽GPC3298-306及GPC3144-152偶联血蓝蛋白(KLH)后免疫Balb/c小鼠,检测免疫后小鼠脾淋巴细胞免疫活性,包括IFN-γ的分泌,以及该淋巴细胞对表达GPC3的鼠肿瘤细胞细胞毒活性。结果经GPC3298-306及GPC3144-152免疫后,小鼠脾细胞中分泌IFN-γ的T淋巴细胞阳性率明显增高(P<0.01);脾淋巴细胞中CD4+T/CD8+T比值亦明显升高(P<0.05);脾淋巴细胞对表达GPC3的鼠肿瘤细胞有特异性的杀伤作用。结论体外表达的GPC3的CTL表位肽能诱导出GPC3特异性的细胞免疫,提示体外表达的GPC3的CTL表位肽GPC3298-306及GPC3144-152可能在在以GPC3为靶向的抗肿瘤免疫治疗中发挥重要作用。Objective To explore whether CTL epitopes GPC3298-306 and GPC3144-152 of Glypican-3 synthesized in vitro can induce GPC3-specific cellular immunity.Methods GPC3298-306 and GPC3144-152 synthesized in vitro were coupled with KLH and inoculated Balb/c mice.Splenocytes was isolated from inoculated Balb/c mice and expression of IFN-γ on splenocytes was detected by ELISPOT,and then the percentage of CD4+ T cell and CD8+ T cell in splenocyte was tested by flow cytometry.Furthermore,the cytotoxicity of splenocytes ...

关 键 词：GLYPICAN-3 细胞免疫 免疫应答 

分 类 号：R392[医药卫生—免疫学]