HPLC法测定肝康灵中大黄素的含量  

Determination of Emodin in Gan Kang Ling Tablets by HPLC

作　　者：余琼[1] 张玉萍[1] 

机构地区：[1]天津中医药大学,天津300193

出　　处：《现代中药研究与实践》2009年第2期37-39,共3页Research and Practice on Chinese Medicines

摘　　要：目的建立HPLC法测定肝康灵中大黄素含量的方法。方法采用HPLC法对制剂中的大黄素进行了定量分析,色谱柱HypersilODS2(250mm×4.6mm),流动相:甲醇-0.1%磷酸(75:25);流速:1.0mL?min-1;检测波长:254nm,柱温30℃。结果大黄素在2.64～10.56mg?mL-1范围内线性关系良好,r=0.9998,平均加样回收率为97.51%,RSD为1.24%(n=6)。结论本方法可将大黄素从复方中充分的提取分离,专属性强,结果准确,可用于肝康灵的质量控制。Objective To establish an HPLC method for the determination of emodin in Gankang Ling tablets. Methods The emodin in Gankang Ling was determined by HPLC. Hypersil ODS2 column(250 mm×4.6 mm) was used, 0.1%phosphoric acid (75:25) was used as the mobile phase. The flow rate was 1.0mL-min-1, The detection wavelength was set at 254 nm, column temperature was at 30℃ . Results The linear range of emodin was 2.64～10.56 mg-mL-1 (r=0.999 8) and the correlation coefficient was 0.999 8, the average recovery rate was 97...

关 键 词：肝康灵 大黄素 高效液相色谱法 含量测定 

分 类 号：R286[医药卫生—中药学]