橡胶树多主棒孢病菌强致病菌株的筛选及产毒条件优化  

Cultural Conditions for Corynespora cassiicola of Heavea brasiliensis

作　　者：彭建华[1] 郑春耀[2] 潘羡心[1] 李超萍[1] 蔡吉苗[1] 黄贵修[1] 

机构地区：[1]中国热带农业科学院环境与植物保护研究所农业部热带农林有害生物入侵监测与控制重点开放实验室,海南儋州5717371 [2]云南天然橡胶股份有限公司勐捧分公司第三作业区

出　　处：《热带作物学报》2009年第4期520-524,共5页Chinese Journal of Tropical Crops

基　　金：农业部公益行业科研专项(nyhyzx07-033-2);中央级公益性科研院所基本科研业务费专项(2008hzs/J007)资助

摘　　要：从采自海南、云南和广西的橡胶树棒孢霉落叶病病样中分离获得17株单孢菌株,致病性测定结果表明,来自海南的HC-10菌株致病性最强。HC-10产毒条件及毒素活性测定结果表明,改良Fries3号培养基、25℃光照12h培养10d、转速为160r/min以及培养基初始pH值为4最有利于毒素的产生;温度、紫外线及不同的毒素浓度与毒素的活性存在一定的相关性。Seventeen isolates were obtained from the Corynespora cassiicola samples collected from rubber tree nurseries and mature trees in Hainan, Yunnan and Guangxi. Of these isolates 3 isolates come from Yunnan, 1 isolate from Guangxi, and the other isolates from Hainan. They were detected in pathogenicity and cultured. Pathogenicity detection showed that Isolate HC-10 from Hainan had the highest pathogenicity, and their culture and crude toxin detection showed that culture of the isolates at 25 ℃ for 10 days at t...

关 键 词：橡胶树 多主棒孢 产毒条件 粗毒素活性 

分 类 号：S76[农业科学—森林保护学]