“桐优1”等泡桐优良无性系组培高效繁育体系建立  

Establishment of High Effect Regeneration System of Tissue Culture for Three Improved Clones in Paulownia fortunei

作　　者：江香梅[1] 戴小英[1] 温强[1] 杨书斌[2] 李秋英[2] 

机构地区：[1]江西省林业科学院省植物生物技术技术重点实验室,江西南昌330032 [2]抚州市林业科学研究所,江西临川344000

出　　处：《江西林业科技》2009年第2期11-14,共4页Jiangxi Forestry Science and Technology

基　　金：江西省科技厅农业重点攻关项目"中;高密度纤维板材造林树种选择与定向培育技术研究"的内容之一(项目编号:20051B0204000)。

摘　　要：采用抚州市林业科学研究所和江西省林业科学院选育、江西省林木良种审定委员会认定的"桐优1"等3个泡桐优良无性系为繁殖材料,对无菌系建立外植体筛选、外植体诱导、分化、增殖、组培苗生根等条件进行了较系统的优化试验,结果表明:以根萌条为外植体是建立无菌系的理想材料;外植体诱导培养基以MS+BA2.0mg/L(单位下同)+NAA0.2+2,4D0.1为好;分化和增殖培养基以MS+BA3.0+IBA0.3;生根培养基以MS+IBA0.1为好。为降低育苗成本,培养基中的碳源用食用白糖(30g/L),固化物用卡拉胶(7g/L)。本试验通过优化筛选,建立了一套经济、高效的组培快速繁殖体系,为泡桐组培规模化生产奠定了良好的物质和技术基础。With three improved clones just as "Tongyou1"et al.select-cultivated by Fuzhou City Forestry Research Institute and Jiangxi Academy of Forestry,convinced by Jiangxi Forest Tree Breeding Examining and Approving Committee for propagation materials,the optimization conditions for explants selection,adventitious bud induction,differentiation,propagation and rooting were tested systematically in this paper.The results demonstrated that the ideal explants materials for sterilization clones construction were shoot...

关 键 词：泡桐 “桐优1” 优良无性系 组培繁殖 

分 类 号：S792.43[农业科学—林木遗传育种]