猪生殖与呼吸综合征病毒JK-100弱毒株生物学和血清学特性研究  

Biological and Serological Characters of Modified Porcine Reproductive and Respiratory Syndrome Virus JK-100 Strain

作　　者：杜文金[1] 张德鸿[2] 王志亮[1] 孙融冰[1] 宋翠平[1] 刘卫[2] 况慧星[2] 

机构地区：[1]农业部动物检疫所核心生物技术实验室,青岛266032 [2]新加坡国立大学分子农业生物学院,新加坡117601

出　　处：《中国预防兽医学报》2000年第S1期37-39,共3页Chinese Journal of Preventive Veterinary Medicine

摘　　要：通过ＩＦＡ、ＶＮＴ和Ｗｅｓｔｅｒｎ－Ｂｌｏｔ等血清学试验及电镜观察，证明ＪＫ－１００株为ＰＲＲＳＶ。本病毒感染ＭＡＲＣ１４５细胞后，３６小时的ＴＣＩＤ_(50)最高，为１０^(-7.83)。把本病毒分别保存在４℃、３７℃各９天，保存在４℃９天后，ＴＣＩＤ_(50)从１０^(-7.83)下降至１０^(-7.32)，３７℃７天已测不到效价。The JK-100 strain was identified as PRRSV by IFA,VNT,Westem-Blot and EM.The TCID50 was highest when MARC145 cell was infected by JK-100 after 36 hours.The virus was conserved under for days, it s titration reduced from 10-7.83 to 10-7.32, and under 37 for 7 days, it s titration can not be detected.

关 键 词：猪生殖-呼吸综合征病毒 JK-100弱毒株 

分 类 号：S85[农业科学—兽医学]