应用PCR检测非洲猪瘟病毒的研究  

Detection of African Swine Fever Virus(ASFV) by Polymerase Chain Reaction (PCR)

作　　者：蒋正军[1] 龚振华[1] 尹燕博[1] 王树双[1] 蔡丽娟[1] 郭福生[1] 陆明哲[1] 孙承英[1] 宣华[2] 

机构地区：[1]农业部动物检疫所,青岛266032 [2]解放军军需大学,长春130000

出　　处：《中国预防兽医学报》2000年第S1期239-242,共4页Chinese Journal of Preventive Veterinary Medicine

摘　　要：采用ＰＣＲ对非洲猪瘟病毒（ＡＳＦＶ）Ｓｐａｉｎ ７０株、Ｍａｌａｗｉ ８３株、Ｍａｌｔａ ７８株、Ｕｇａｎｄａ株的细胞培养毒以及ＡＳＦＶ组织病料和 ２００份田间样品进行检测，结果表明两对引物 Ｐ１＋ Ｐ２和 Ｐ３＋ Ｐ４对 ＡＳＦＶ具有较好的特异性和灵敏性。运用 ＰＣＲ检测 ＡＳ－ＦＶ操作简单、快速，检测结果与ＨＡＤ自动玫瑰花环试验的阳性符合率为１００％，该方法明显优于常规的血清学方法。Two pairs of primers, P1 + P2and P3 + P4were used to detect the ASFV by PCR. The cultured ASFV strains Spain70, Malawi 83, Malta 78 and Uganda as well as 200 histological materials were detected by these two pairs of primers. The positive detection results were in consistence with HAD to 100%. The results demonstrated that this method was sensitive and easy to be operated. The PCR is a better way compared with other methods used for the detection of ASFV.

关 键 词：非洲猪瘟病毒 PCR 

分 类 号：S85[农业科学—兽医学]