新城疫病毒的分离与分子生物学分析  

Isolation and Genotyping of Newcastle Disease Virus

作　　者：王志亮[1] 杜文金[1] 孙振涛[2] 单良刚[3] 郑东霞[1] 张子春[1] 宋翠平[1] 孙融冰[1] 况慧星[4] 

机构地区：[1]农业部动物检疫所核心生物技术实验室,青岛266032 [2]山东聊城东昌府区畜牧局 [3]山东泗水粮食局 [4]新加坡国立大学分子农业生物学院

出　　处：《中国预防兽医学报》2000年第S1期69-71,共3页Chinese Journal of Preventive Veterinary Medicine

摘　　要：我们从１９９８－２０００年期间分离的多个毒株中，选取了１个鸽源和在沿海及西北等不同地区分离的３个鸡源毒株，从血清学、毒力学到分子生物学等方面进行了全面分析。从这些毒株对１日龄ＳＰＦ鸡的致病指数来看，鸽源毒株对鸡的毒力为中发型，而鸡源毒株的毒力均为速发型。Ｆ基因序列分析表明，３个鸡源毒株的同源性为９８％，与台湾１９９８年分离株的同源性为９５％，都是基因Ⅶ型。鸽源毒株与鸡源毒株的同源性为 ８９％，属基因 ＶＩ型。所有分离毒与 Ｌａ Ｓｏｔａ疫苗株和 Ｆ４８Ｅ９的同源性仅为８２－８４％。初步认为目前引起鸡发病的毒株主要为基因Ⅶ型。Four Newcastle disease virus strains, one from pigeon and the others from vaccinated chicks, were isolated in recent outbreaks in China. Intracerebral pathogenicity index test suggested that the pigeon strain was mesogenic while all the chicken isolates were velogenic. The results of the F gene sequence analysis showed that the chicken isolates had a similarity of 98% to each other and that of 89% to the pigeon isolate. Alignment of the deduced amino acid sequences with published sequences in Genebank...

关 键 词：新城疫病毒 脑内致病指数 基因型 序列分析 

分 类 号：S85[农业科学—兽医学]