间接免疫荧光抗体法对鸡传染性支气管炎病毒进行抗原定位  

Localization of Avian Infectious Bronchitis Virus by Indirect Immuno - Fluorescent Antibody Plan

作　　者：刘思国[1] 尹训南[1] 李广兴[2] 康丽娟[1] 江国托[1] 孔宪刚[1] 刘忠贵[2] 卢景良[1] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室,哈尔滨150001 [2]东北农业大学动物医学院

出　　处：《中国预防兽医学报》2000年第S1期82-85,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家攀登计划B类项目!"85-44-01-44"

摘　　要：应用间接免疫荧光抗体（ＩＦＡ）法对ＩＢＶ感染后ＳＰＦ鸡不同脏器进行了跟踪检测。结果发现，在ＩＢＶ感染后的第７天，在气管、肾脏、脾脏、肺脏和肝脏等组织细胞中均不同程度地出现特异性的荧光。表明在感染的初期，ＩＢＶ具有广泛的组织嗜性；在感染的第１０天，只有肾脏、气管或肺脏中呈现荧光，而以肾脏中的荧光最强，特异性荧光持续时间最长的是肾脏，可达１４天，病毒存在于肾小管间质内浸润的淋巴细胞和巨噬细胞胞浆中。实验结果证实，ＩＦＡ方法具有直观、特异等特点，在抗原定位上具有独特的优点。IFA was used to detect differene organs of SPF chickens infected IBV. Result showed that there were differene degree specific immuno- fluonrecent in cells of trachea, kidney, spleen, lung and liver. It showed that IBV has broad tissue tropism early period of infection. At 10 days post infection, there was light in only kidney and lung, and the light is most noticable in kidney. The lasting time of immuno-flu- onrecent in kidney can last 14 days and is loger than others. Virus was in lymphocytes and macrop...

关 键 词：鸡传染性支气管炎病毒 间接免疫荧光抗体法 抗原定位 

分 类 号：S85[农业科学—兽医学]