不同地理株伊氏锥虫灭活苗交叉免疫保护及免疫方法研究  

Cross Immune Protection and Immune Methods of Inactivated Trypanosoma evansi Vaccinesfrom Different Geography Strains

作　　者：王祥生[1] 王德昭[1] 刘全[1] 陈林[1] 王黎煜[1] 何畅[1] 

机构地区：[1]解放军军需大学军事兽医研究所,长春130062

出　　处：《中国预防兽医学报》2000年第S1期157-160,共4页Chinese Journal of Preventive Veterinary Medicine

摘　　要：用伊氏锥虫湖北株和广西株制备纯虫灭活苗和含血灭活苗，经小鼠１次、２次免疫，再经强毒伊氏锥虫湖北株、广西株和江苏株交叉攻击。结果湖北株纯虫灭活苗２次免疫鼠，对同株（湖北株）获得３０／３０保护，对异株即广西株和江苏株分别获０／１０和７／１０保护，该苗一次免疫鼠对同株仅获得５／１０保护。广西株纯虫灭活苗２次免疫鼠对同株（广西株）获２０／２０保护，对异株即湖北株和江苏株分别获得０／１０和４／１０保护。含血湖北株伊氏锥虫灭活苗对同株无保护作用，１３只免疫小鼠全部死亡，对江苏株（异株）为９／１０死亡。对照小鼠全部发病死亡。交叉免疫保护试验结果说明，纯虫灭活苗经两次免疫后对伺株虫体产生坚强的免疫保护作用，免疫保护率达１００％，不同地理株伊氏锥虫因抗原变异现象出现不同的免疫保护作用。两次免疫接种小鼠其免疫保护效果比一次免疫的效果好。疫苗中如含有大量非特异物质，虫苗的免疫保护作用将完全丧失。稳定试验表明，灭活苗在室温下保存１个月，在４℃、－２０℃下保存６个月，免疫效果不变。The inactivated pure parasite vaccine and the vaccine contained mouse blood were prepared with Trypanosoma evansi of Hubei and Guangxi strains in vitro cultivation respectively. The mice were challenged with natural strains of Hubei, Guangxi and Jiangshu T. evansi after they were inoculated by 1 or 2 times. The results showed that 30/30 of the mice inoculated by 2 times with Hubei strain pure parasite vac- cine were protected from the same strain(Hubei strain), however the immune mice challenged w...

关 键 词：伊氏锥虫 灭活苗 交叉免疫保护 小鼠 

分 类 号：S85[农业科学—兽医学]