利用SMART技术构建白菜型油菜cDNA文库  

cDNA LIBRARY CONSTRUCTION OF B.CAMPESTRIS WITH SMART OLIGO

作　　者：袁旭[1] 王劲[1] 李旭峰[1] 

机构地区：[1]四川大学生命科学学院植物遗传室,成都610064

出　　处：《四川大学学报（自然科学版）》2000年第S1期72-75,共4页Journal of Sichuan University(Natural Science Edition)

摘　　要：作者用SMART技术构建了芸苔属三个基本种之一———白菜型油菜的cDNA文库 .所构建的cDNA文库滴度为 1.0 2× 10 6 pfu/mL ,重组率为 82 % .扩增后文库的滴度为 7×10 12 pfu/mL .利用PCR方法扩增插入片段 ,其长度在 50 0bp～ 1.5kb之间 .Using SMART Oligo, the authors constructed a cDNA library of B.campestris to isolate the cDNA of RAD51, a RecA like gene, which is essential along with other genes for mitotic and meiotic recombination and DNA repair. The cDNA library consist of 1.02×10 6 clones. By PCR reaction the authors amplified the inserts and found the size of the inserts were between 500bp and 1.5kb.

关 键 词：白菜型油菜 CDNA文库 SMART技术 

分 类 号：Q785[生物学—分子生物学]