越冬松针瘿蚊幼虫整体携脂蛋白的分离和纯化  

Purification and characterization of lipophorin from overwintering mature larvae of pine needle gall midge, Thecodiplosis japonensis (Diptera:Cecidomiidae)

作　　者：李毅平[1] 龚和[2] 朴镐用 

机构地区：[1]中国科学院动物研究所农业虫鼠害综合治理研究国家重点实验室,北京100080 [2]中国科学院动物研究所 [3]韩国科学技术院生命工学所昆虫资源室,韩国大田305600

出　　处：《昆虫学报》2000年第S1期77-84,共8页Acta Entomologica Sinica

基　　金：中韩国际合作项目;农业虫鼠害综合治理研究国家重点实验室资助课题

摘　　要：采用KBr密度梯度超速离心并结合常规SepharoseCL 4B凝胶柱层析 ,从越冬松针瘿蚊Thecodiplosisjaponensis (UchidaetInouye)幼虫整体中 ,分离并纯化了一种携脂蛋白。这是第二例从昆虫整体分离并纯化出携脂蛋白的报道。采用凝胶柱层析确定该携脂蛋白的相对分子质量为6 38kD ,它是由分别为 2 40kD和 5 2kD的两个亚基组成。整体分子中含有 5 2 8%的蛋白和47 2 %的脂类。苏丹黑B和希夫氏试剂染色显示阳性 ,说明它是一种糖脂复合蛋白。采用超速离心确定它的密度为 1 11g/mL 。A lipophorin was isolated and purified from whole body of third instar mature larvae of pine needle gall midge, Thecodiplosis japonensis (Uchida et Inouye) by KBr density gradient ultracentrifugation and Sepharose CL 4B gel permeation. It is the second lipophorin that have been purified from whole body. The native molecular weight was 638 kD by gel permeation chromatography. It was composed of two subunits, named as apoLp Ⅰ and apoLp Ⅱ, with molecular weight of 240 kD and 52 kD respectively. The ho...

关 键 词：日本松针瘿蚊 携脂蛋白 高密度脂蛋白 越冬幼虫 

分 类 号：Q966[生物学—昆虫学]