高压氧与常压氧治疗一氧化碳中毒昏迷大鼠的实验性对比研究  

Hyperbaric Oxygen Comparing with Normobaric Oxygen in the Treatment of Carbon Monoxide Poisoning Rat

作　　者：王燕[1] 刘郁[1] 贾辉[1] 陈学新 尚桂菊 郝鸣政 

机构地区：[1]中国医科大学第一临床学院急诊科,沈阳110001

出　　处：《中国医科大学学报》2000年第S1期44-45,共2页Journal of China Medical University

摘　　要：目的 :观察高压氧 ( hyperbaric oxygen,HBO)、常压氧 ( normobaric oxygen,NBO)治疗前后一氧化碳中毒昏迷大鼠小脑组织中 SOD、MDA的变化。方法 :建立实验性 CO中毒昏迷大鼠模型 ,采用羟胺氧化法、硫代巴比妥酸比色法分别测定各组大鼠小脑组织匀浆中 SOD、MDA的水平。结果 :HBO治疗有效恢复了小脑中 MDA水平 ,HBO3次治疗效果更好。 HOB1次治疗和 3次治疗均有效地恢复了 SOD活性 ;NBO治疗对 SOD活性、MDA的作用不显著。结论 :HBO治疗拮抗了 CO中毒致大鼠小脑组织中的脂质过氧化反应 ,而 NBO治疗无此作用。Objective:The purpose of this study was to evaluate the difference between hyperbaric oxygen(HBO) and normobaric oxygen(NBO) when they were used to treat carbon monoxide poisoning rats by the levels of SOD and MDA in rat cerebellum. Methods:Eighty male Wistar rats were randomly divided into 5 groups. The rats were killed after different therapies. Cerebellum tissues were seperated. We measured SOD and MDA with spectrophotometry. Results:The level of SOD increased and the level of MDA decreased after HBO the...

关 键 词：一氧化碳中毒 小脑组织 高压氧 常压氧 超氧化物歧化酶 脂质过氧化物 

分 类 号：R459.7[医药卫生—急诊医学]