pH敏感荧光探剂BCECF  

A pH-sensitive Fluorescein Dye BCECF

作　　者：熊玉涛[1] 

机构地区：[1]五邑大学学报编辑部,广东江门529020

出　　处：《五邑大学学报（自然科学版）》2000年第3期63-67,共5页Journal of Wuyi University(Natural Science Edition)

基　　金：国家自然科学基金!(29773015)

摘　　要：２’，７’－二（羧乙基）－５（６）－羧基荧光黄（ＢＣＥＣＦ）是一种新型的ｐＨ荧光探剂，它在４４０ｎｍ、４９０ｎｍ两处的荧光强度的比值（ＦＩＲ）与ｐＨ值相关。体外实验证明，ｐＨ值６．２０～７．８０范围内，ＦＩＲ与ｐＨ呈线形关系。用ＢＣＥＣＦ标记的蟾蜍膀胱上皮细胞，在ｐＨ值６．３０～７．７０范围内，细胞内ｐＨ（ｐＨｉ）与ＦＩＲ呈线性关系。用该法首次测得蟾蜍膀胱上皮细胞ｐＨｉ＝７．２１（ｎ＝８６）。bis (carboxyethyl ) -5 (and 6) -carboxy- fluorescein (BCECF) is widely used as pH-sensitive fluorescein probe recently. When BCECF excites at 490mn and 440nm, the ratio of fluorescein intensities (FIR) using the two excitation wavelengths yields a pH-dependent parameter. As a result, FIR is a linear function of pH between pH value 6.20～7.80 ex vivo. Loading toad urinary bladder with BCECF, a similar linear function is resulted between pH value 6.30～7.70. The intracellular pH value of toad urinary bladder we first got is 7.21 (n=86).

关 键 词：BCECF 蟾蜍膀胱 上皮细胞 细胞内pH 

分 类 号：Q73[生物学—分子生物学] O657.3[理学—分析化学]