ABC法显示大鼠脑内nNOS免疫阳性神经元  

Application of ABC immunocytochemistic technique to display of nNOS-immunoreactive neurons in rat brain

作　　者：郭国庆[1] 沈伟哉[1] 肖计划[2] 许丽宾[1] 

机构地区：[1]暨南大学医学院解剖学教研室,广东广州510630 [2]暨南大学医学院附属第一医院

出　　处：《新乡医学院学报》2000年第6期407-409,共3页Journal of Xinxiang Medical University

基　　金：国家自然科学基金! (395 70 2 6 7);广东省医学科研基金! (A19982 90;A19992 81)资助

摘　　要：目的　为进一步研究脑内神经源型NO合酶 (nNOS)免疫阳性神经元的表达提供可靠的实验方法。方法　ABC免疫细胞化学方法。结果　大鼠大脑皮质、尾壳核、海马、中脑、延髓、小脑等中枢神经系统的各个部位均有nNOS免疫阳性神经元和神经末梢出现 ,nNOS免疫阳性神经元胞体呈棕褐色 ,细胞核处颜色浅淡 ,突起有一个或多个 ;nNOS免疫阳性神经纤维呈棕色串珠状 ,且交错分布。结论　ABC法是显示NOS亚型nNOS表达的可靠方法 ,但一抗浓度不宜过高 ,而且需延长抗体孵育的时间。Objective To supply a credible experimental method for study the expression of nNOS-immunoreactive neurons in rat brain. Methods The ABC immunocytochemistic technique was used in brain tissues of 12 rats. Results The nNOS-immunoreactive neurons and innervations appeared in the rat brain, such as cerebral cortex, caudate putamen, hippocampus, midbrain, medullaery bulbus and cerebellum. The nNOS-immunoreactive neurons was brown, the color of nucleus was weak and the bodies of nNOS-immunoreactive neurons had one or more protuberances. The nNOS-immunoreactive innervations exhibited brown strings beadings and had an interlacing distribution. Conclusions The ABC method is credible for study of the expression of nNOS-immunoreactive neurons, at the same time, the moderate concentration of the first antibody is advisable and the time of incubation should be prolonged.

关 键 词：神经元 一氧化氮合酶 脑 免疫细胞化学 大鼠 

分 类 号：R322[医药卫生—人体解剖和组织胚胎学] R322.8[医药卫生—基础医学]